Science Note
[May 9, 2023] Previous Science Note
Mitochondrial unfolded protein response in neural stem cell aging
This article focusing on the mitochondrial protein folding stress is particularly significant in activated neural stem cells (NSCs) and neural progenitor cells (NPCs) within the neurogenic niche. This stress increases with age, leading to dysregulated cell cycles and mitochondrial activity in activated NSCs and NPCs in the dentate gyrus. | |||||
The mitochondrial unfolded protein response regulates hippocampal neural stem cell aging Chin-Ling Wang, et. al., Cell Metabolism (2023) Point of Interest |
|||||
Related Techniques | |||||
Glycolysis/Oxidative phosphorylation assay | Glycolysis/OXPHOS Assay Kit | ||||
Oxygen consumption rate assay | Extracellular OCR Plate Assay Kit | ||||
Mitochondrial membrane potential detection | JC-1 MitoMP Detection Kit / MT-1 MitoMP Detection Kit | ||||
Mitochondrial superoxide detection | mtSOX Deep red - Mitochondrial superoxide Detection | ||||
Mitophagy Detection | Mitophagy Detection Kit and Mtphagy Dye | ||||
Cellular senescence detection (Live cell imaging or FCM) | Cellular Senescence Detection Kit | ||||
Cellular senescence detection (Plate reader) | Cellular Senescence Plate Assay Kit | ||||
Lysosomal function assay | Lysosomal pH and mass detection Kit | ||||
Related Applications | |||||
Metabolic shift to glycolysis in senescenct cells
|
|||||
2. Simultaneously detection of Lysosomal and Mitochondrial DysfunctionWe tried the simultaneous detection of lysosomal and mitochondrial dysfunction in Hela cells treated with CCCP or Antimycin (AN). CCCP and AN are well-known inducers of mitochondrial ROS regarding loss of mitochondrial membrane potential. Recent research showed the result that CCCP induces not only mitochondrial ROS but also lysosomal neutralization. To detect mitochondrial ROS, HeLa cells were labeled by mtSOX Deep Red - Mitochondrial Superoxide Detection, and the lysosomal mass and pH were detected separately with LysoPrime Green and pHLys Red. Co-staining with mtSOX and Lysosomal dyes demonstrated that CCCP causes lysosomal neutralization and mitochondrial ROS induction at the same time. Products in Use
|