Reagent for Monitoring Calcium Ion
Product codeR002 Rhod 2-AM
Chemical name1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, tetraacetoxymethyl ester, bromide
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Rhod 2 has the longest fluorescent wavelength signal of all the calcium indicators. It has a rhodamine-like fluorophore whose excitation and emission maxima are 557 nm and 581 nm, respectively. This makes it a convenient excitation source for argon and krypton lasers. Although it was thought that the fluorescent signal of Rhod 2 temp-only increases several times with the calcium complex, Dojindo’s Rhod 2 increases its signal with calcium 80-100 times because of its high purity. Its signal intensity is the strongest of all the calcium probes. Rhod 2 is thus highly recommended as a probe for intracellular calcium monitoring using laser microscopes. Rhod 2 is reported to have a better loading profile at the point of localization, especially in neural slice cultures. The dissociation constant of Rhod 2 with calcium (Kd=1.0 mM) is the highest of all the fluorescent calcium probes, providing a wider range for monitoring calcium concentration. Rhod 2-AM is an acetoxymethyl ester derivative of Rhod 2 that can be easily loaded into cells using the AM method.
1) M. Burnier, G. Centeno, E. Burki and H. R. Brunner, "Confocal Microscopy to Analyze Cytosolic and Nuclear Calcium in Cultured Vascular Cells", Am. J. Physiol., 1994, 266, C1118.
2) J. Vergara, M. Difranco, D. Compagnon and B. A. Suarez-Isla, "Imaging of Calcium Transients in Skeletal Muscle Fibers", Biophys. J., 1991, 59, 12.
3) T. Meyer, T. Wensel and L. Stryer, "Kinetics of Calcium Channel Opening by Inositol 1,4,5-Trisphosphate", Biochemistry, 1990, 29, 32.
4) A. Minta, J. P. Y. Kao and R. Y. Tsien, "Fluorescent Indicators for Cytosolic Calcium Based on Rhodamine and Fluorescein Chromophores", J. Biol. Chem., 1989, 264(14), 8171.
5) D. R. Trollinger, W. E. Cascio and J. J. Lemasters, "Selective Loading of Rhod 2 into Mitochondria Shows Mitochondrial Ca2+ Transients during the Contractile Cycle in Adult Rabbit Cardiac Myocytes", Biochem. Biophys. Res. Commun.,> 1997, 236, 738.
Handling and storage condition
|Appearance：||Dark purple solid|
|Fluorescence spectrum：||To pass test|
|-20°C, Protect from light|