Exploring Ferroptosis Resistance Mechanisms for Cancer Treatment [Oct. 29, 2024]

[Oct. 29, 2024] Previous Science Note

In recent cancer research, ferroptosis, a type of regulated cell death involving iron-dependent lipid peroxidation, has shown promise in cancer treatment. Here are some of the papers that have identified mechanisms of ferroptosis resistance in cancer cells and suggested that they may be therapeutic targets.

Ferroptosis, a type of regulated cell death involving iron-dependent lipid peroxidation, has shown promise in cancer treatment. It provides an alternative pathway to eliminate cancer cells that are resistant to traditional therapies such as apoptosis. By targeting ferroptosis, researchers can exploit metabolic weaknesses in cancer cells, particularly when antioxidant defenses are disrupted. This approach is being explored to improve the efficacy of existing treatments and potentially overcome drug resistance in several types of cancer.

7-Dehydrocholesterol is an endogenous suppressor of ferroptosis
Click here for the original article: Florencio Porto Freitas, et. al., Nature, 2024.

Point of Interest

- High concentrations of 7-DHC are cytotoxic to developing neurons by promoting lipid peroxidation.

- On the other hand, 7-DHC accumulation confers a prosurvival function in cancer cells.

- 7-DHC effectively protects (phospho)lipids from autoxidation and subsequent fragmentation due to its superior reactivity with peroxyl radicals.

- Accumulation of 7-DHC induces a shift to a ferroptosis-resistant state in tumors.

Tumor suppressor Par-4 activates autophagy-dependent ferroptosis
Click here for the original article: Karthikeyan Subburayan et. al., Communications Biology, 2024.

Point of Interest

- Point o- Par-4/PAWR activation is critical during ferroptosis, with its depletion blocking and overexpression enhancing this ferroptosis process.

- Upregulation of Par-4 promotes NCOA4-dependent ferritinophagy, a selective autophagy process that degrades ferritin, increasing free iron release, lipid peroxidation, and ROS production.

- Par-4 inhibition blocks ferroptosis-driven tumor suppression, highlighting its potential for cancer therapy.

BRCA1-Mediated Dual Regulation of Ferroptosis Exposes a Vulnerability to GPX4 and PARP Co-Inhibition in BRCA1-Deficient Cancers
Click here for the original article: Jonathan Sun et. al., Cancer Discovery, 2024.

Point of Interest

- BRCA1 deficiency in cancers confers resistance to erastin-induced ferroptosis but increases susceptibility to GPX4 inhibitor-induced ferroptosis.

- Combined PARP and GPX4 inhibition in BRCA1-deficient cancers promotes ferroptosis, providing a strategy to overcome PARP inhibitor resistance.

- Reduced GPX4 expression and NCOA4-induced ferritinophagy in BRCA1-mutant cancers increases sensitivity to co-treatment with PARP and GPX4 inhibitors.

Related Techniques

Intracellular / mitochondrial ferrous ion (Fe2+) detection
FerroOrange(intracellular), Mito-FerroGreen(mitochondrial)
Cellular senescence detection
SPiDER-βGal for live-cell imaging or flow cytometry / microplate reader / tissue samples
SPiDER-βGal Blue for fixed cell and for multiple staining with immunostaining and other methods
Lipid peroxidation detection
Liperfluo(intracellular), MitoPeDPP(mitochondrial)
Lipid Peroxidation Assay
Lipid Peroxidation Probe -BDP 581/591 C11-
Total ROS detection
Highly sensitive DCFH-DA or Photo-oxidation Resistant DCFH-DA
Mitochondrial superoxide detection
MitoBright ROS Deep Red - Mitochondrial Superoxide Detection
Glutathione Quantification
GSSG/GSH Quantification Kit
Cystine Uptake detection
Cystine Uptake Assay Kit
MDA detection
MDA Assay Kit
First-time autophagy research
Autophagic Flux Assay Kit
Glycolysis/Oxidative phosphorylation Assay
Glycolysis/OXPHOS Assay Kit
Apoptosis detection in multiple samples
Annexin V Apoptosis Plate Assay Kit
Mitochondrial membrane potential detection
JC-1 MitoMP Detection KitMT-1 MitoMP Detection Kit
Cell proliferation/ cytotoxicity assay
Cell Counting Kit-8 and Cytotoxicity LDH Assay Kit-WST

Related Applications

Erastin-Induced Ferroptosis: Evaluating Intracellular Uptake and Redox Balance

We investigated the transition of cellular metabolisms in A549 cells treated with erastin, a known ferroptosis inducer. Our results revealed the following.

 

- The inhibition of cystine uptake by erastin led to a depletion of cysteine, which in turn increased the compensatory uptake of other amino acids.
- Glucose uptake, which typically promotes ferroptosis*, was found to decrease upon erastin treatment, suggesting a potential cellular self-defense mechanism.
- The depletion of cysteine resulted in a decrease in glutathione levels and an increase in Fe2+, ROS, and lipid peroxides, all of which are recognized markers of ferroptosis.


  Cell Line: A549
  Incubation Conditions: 100 μmol/l Erastin/MEM, 37℃, 3h
  *Reference: Xinxin Song, et al., Cell Reports, (2021)

Products in Use

① Amino Acid Uptake Amino Acid Uptake Assay Kit
② Glucose Uptake Glucose Uptake Assay Kit-Green
③ Cystine Uptake : Cystine Uptake Assay Kit
④ Intracellular glutathione GSSG/GSH Quantification Kit
⑤ Intracellular labile Fe FerroOrange
⑥ Intracellular total ROS ROS Assay Kit -Highly Sensitive DCFH-DA-
⑦ Lipid Peroxides Liperfluo

 

Product Classification

Product Classification