MDA Assay Kit

MDA Assay Kit

MDA(malondialdehyde) Assay Kit

  • Capable of measuring MDA in cells and tissues
  • Simple procedure
  • Product code
    M496  MDA Assay Kit
Unit size Price Item Code

<Approximate number of uses> One plate of 96-well plate

Component

Description

 Malondialdehyde (MDA) is one of the end products of lipid peroxides, the MDA concentration in cell or tissue samples can indicate lipid peroxidation which is important in the fields of oxidative stress and ferroptosis. MDA is also called reactive aldehyde and reacts with amino and thiol groups to cause protein denaturation and DNA damage, so it is also measured in various disease studies such as cancer and diabetes.
 Dojindo's MDA Assay Kit uses the TBARS method to detect MDA by measuring the fluorescence or absorbance of the adduct of MDA and thiobarbituric acid that develops color according to the amount of MDA. The kit also contains MDA standard solution, which can be used to prepare a standard curve and quantify the MDA concentration in the sample.

Manual

Q & A

Q

What is the principle of this kit?

A

This kit uses 2-thiobarbituric acid(TBS) as a substrate. By measuring the absorbance or fluorescence intensity of the TBA adduct formed by the reaction of MDA and TBA, and comparing with the standard curve, the MDA concentration in the sample can be simply quantified. 

Q

How many samples can be tested by this kit?

A

When testing the sample in triplicates, 24 samples can be tested by this kit.

Q

What is the storage condition of the prepared solution?

A

Substrate stock solution is stable for 2 months under -20℃.
Antioxidant PBS solution and Working solution is stable for 24 hours.

Q

Do I need to normalize the result by cell numbers?

A

If there is no change in cell number after drug stimulation, the normalization by cell number is not necessary.
If the number of cells changes, the normalized result by protein quantification is more accurate, in such case, the BCA method is recommended for protein quantification.

Q

What is the LOQ (Limit of Quantification) of this kit?

A

The limit of quantification of both the fluorometric method and the colorimetric method of MDA is 1 µmol/l.

Handling and storage condition

Handling and storage condition
1. Hazardous material Class 4, Petroleums No. 2, Hazardous material Class III, Petroleums No. 3, Hazardous material Class III, 2. Health and Safety Law, Chemical Substances Control Law, 3. Prevent from fire. 4. Store in a deep freeze at -20℃.
PRTR Policy Class 1 designated chemical substance
Danger / harmful
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