JC-1 MitoMP Detection Kit
Mitochondrial Membrane Potential Detection
Product codeMT09 JC-1 MitoMP Detection Kit
|Unit size||Price||Item Code|
|1 set||JC-1 Dye
Imaging Buffer (10x)
|100 nmol x1
11 ml x1
Mitochondria synthesize ATP using oxygen to produce necessary energy for living cells. Lowering of mitochondrial activity and dysfunction are known to be closely related to cancer, aging, and neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases. Mitochondrial membrane potential is a parameter used to measure with mitochondrial condition.
JC-1 forms aggregate (in healthy mitochondria) with red fluorescence. As membrane potential decreases, JC-1 becomes monomers, which shows in green fluorescence. The change in ratio of red to green fluorescence is used as a indicator of mitochondrial condition.
Easy to Use
Easy to dissolve
JC-1 has been difficult to dissolve, but this kit solves the problem!
Detect by Several Equipments
Please refer to Data: Induced Apoptosis for experimental examples
Imaging Buffer Included
HeLa cells treated with depolarizing reagent, carbonylcyanide-p-trifluoromethoxyphenylhydrazone (FCCP) were stained with JC-1 MitoMP Detection Kit. Red fluorescence indicates normal membrane potential or health mitochondria. Untreated cells showed red fluorescence, while FCCP treated cells showed little red fluorescence.
Data: Induced Apoptosis
Jurkat cells treated by apoptosis inducing reagent, Staurosporine, were stained with JC-1 MitoMP Detection Kit. Procedures for these data can be found in the Technical Manual.
Fluorescence imaging of mitochondrial membrane potential in Jurkat cells
Flow cytometric analysis of mitochondrial membrane potential in Jurkat cells
Fluorescence intensity ratio of mitochondrial membrane potential in Jurkat cells
Required amount of Imaging Buffer solution by vessel type
Mitophagy Induction and Detection of Mitochondrial Membrane Potential Changes
Mitochondrial condition in the carbonyl cyanide m-chlorophenyl hydrazine (CCCP) treated Parkin-expressing HeLa cells was compared with untreated cells using Mitophagy Detection Kit (MD01, MT02) and JC-1 MitoMP Detection Kit (MT09).
Mitophagy was not detected in untreated cells and the membrane potential was normal. However, reduction of membrane potential and mitophagy were observed in treated cells.
Ex: 561 nm, Em: 570-700 nm
[Mitochondrial Membrane Potential Detection]
Green Ex: 488 nm, Em: 500-550 nm
Red Ex: 561 nm, Em: 560-610 nm
|Fluorescent Microscope||T. Namba, "BAP31 regulates mitochondrial function via interaction with Tom40 within ER-mitochondria contact sites ", Sci Adv., 2019, 5, (6), 1386.|
|Fluorescent Microscope||I. Kawahata, L. Luc Bousset, R. Melki and K. Fukunaga, "Fatty Acid-Binding Protein 3 is Critical for α-Synuclein Uptake and MPP+-Induced Mitochondrial Dysfunction in Cultured Dopaminergic Neurons ", Int J Mol Sci., 2019, 20, 5358.|
|Plate Reader||M. Kurano, K. Tsukamoto, T. Shimizu, H. Kassai, K. Nakao, A. Aiba, M. Hara and Yatomi, "Protection Against Insulin Resistance by Apolipoprotein M/Sphingosine 1-Phosphate ", Diabetes, 2020, DOI: 10.2337/db19-0811.|
|Plate Reader||T. Nechiporuk, S.E. Kurtz, O. Nikolova, T. Liu, C.L. Jones, A. D. Alessandro, R. C. Hill, A. Almeida, S. K. Joshi, M. Rosenberg, C. E. Tognon, A. V. Danilov, B. J. Druker, B. H. Chang, S. K McWeeney and J. W. Tyner, "The TP53 Apoptotic Network Is a Primary Mediator of Resistance to BCL2 Inhibition in AML Cells.", Cancer Discov, 2019, 9,|
|Fluorescent Microscope||G. Yang, M. Fan, J. Zhu, C. Ling, L. Wu, X. Zhang, M. Zhang, J. Li, Q. Yao, Z. Gu and X. Cai, "A multifunctional anti-inflammatory drug that can specifically target activated macrophages massively deplete intracellular H2O2 and produce large amounts CO for a highly efficient treatment of osreoarthritis", Biomaterials, 2020, doi:10.1016/j.biomaterials.2020.120155.|
|Fluorescent Microscope||J. H. Quan, F. F. Gao, H. A. Ismail, J. M. Yuk, G. H. Cha, J. Q. Chu and Y. H. Lee, "Silver Nanoparticle-Induced Apoptosis in ARPE-19 Cells Is Inhibited by Toxoplasma gondii Pre-Infection Through Suppression of NOX4-Dependent ROS Generation", Int J Nanomedicine, 2020, 15, 3695–3716.|
|Flow Cytometer||C. N. D’Alessandro-Gabazza, T. Yasuma, T. Kobayashi, M. Toda1, A. M. Abdel-Hamid, H. Fujimoto, O. Hataji, H. Nakahara, A. Takeshita, K. Nishihama, T. Okano, H. Saiki, Y. Okano, A. Tomaru, V. F. D’Alessandro, M. Shiraishi, A. Mizoguchi, R. Ono, J. Ohtsuka, M. Fukumura, T. Nosaka, X. Mi, D. Shukla, K. Kataoka, Y. Kondoh, M. Hirose, T. Arai, Y. Inoue, Y. Yano, R. I. Mackie, I. Cann and E. C. Gabazza, "Inhibition of lung microbiota-derived proapoptotic peptides ameliorates acute exacerbation of pulmonary fibrosis", Nat. Comm., 2022, doi:10.1038/s41467-022-29064-3.|
Handling and storage condition