Abrogated CBFb activity induces senescence-like phenotypes in macrophages DOJINDO LABORATORIES

Previous Science Note

Through single-cell RNA sequencing (scRNA-seq), they identified an indispensable transcription factor, CBFb that ensures AM self-renewal. The study concludes that AMs in aged hosts exhibit signs of senescence, possibly due to reduced activity of the CBFb transcription factor.
Single cell RNA sequencing unravels mechanisms underlying senescence-like phenotypes of alveolar macrophages Click here for the original article: Yue Wu, et. al., iScience, 2023.
Point of Interest - Damaged and aged alveolar macrophages (AMs) showed reduced embryonic stem-cell-like features and compromised DNA repair abilities, respectively. - These deficiencies were associated with a decrease in self-renewal capabilities and cellular senescence. - AMs in aged hosts exhibit signs of senescence, possibly due to reduced activity of the CBFb transcription factor.
Related Techniques
First choice for cellular senescence assay	Cellular Senescence Detection Kit – SPiDER-ßGal
Cellular senescence assay with a plate reader	Cellular Senescence Plate Assay Kit – SPiDER-ßGal
Cell cycle assay	Cell Cycle Assay Solution Blue / Deep Red
Dead cell staining for flow cytometry	Dead Cell Makeup Blue / Deep Red - Higher Retention than PI
Lysosomal pH detection	Lysosomal Acidic pH Detection Kit-Green/Red, Green/Deep Red
Mitochondrial function/glycolysis detection	Glycolysis/JC-1 MitoMP Assay Kit
Oxygen consumption rate assay	Extracellular OCR Plate Assay Kit

Related Applications
Analysis of Lysosomal Mass and pH Exchange in Senescence-induced Cells
		Purpose: To investigate changes in lysosomal mass and pH in A549 cells induced to senescence by treatment with Doxorubicin (DOX). Methods: Senescence-associated acidic β-galactosidase (SA-βGal) activity was detected using Cellular Senescence Detection Kit - SPiDER-βGal. Lysosomal mass was detected using LysoPrime Deep Red, and pH was detected using pHLys Red. Fluorescence imaging was used to observe changes in lysosomal mass and pH in senescent cells compared to non-senescent cells. The normalized fluorescence intensity of lysosomal mass and pH was also measured by a plate reader. Results: Our findings indicate that senescence induced by DOX resulted in an increase in lysosomal mass and acidification of pH compared to non-senescent cells. The obtained results are consistent with previous reports* that demonstrated enhanced lysosomal activity in senescent cells induced by the CDK4/6 inhibitor, palbociclib. The fluorescence imaging and plate reader data both support these findings. * Miguel Rovira, et. al., Aging Cell (2022) ＜Experimental Conditions for Microscopy＞ SA-βGal(Green):Ex = 488 nm, Em = 490 – 550 nm Lysosomal pH (Red):Ex = 561 nm, Em = 560 – 620 nm Lysosomal mass (Deep Red):Ex = 633 nm, Em = 640 – 700 nm ＜Experimental Conditions for Plate Reader＞ SA-βGal: Ex = 525 – 535 nm, Em = 550 – 570 nm Lysosomal pH: Ex = 555 – 565 nm, Em = 590 – 610 nm Lysosomal mass: Ex = 645 – 655 nm, Em = 690 – 710 nm <Products in Use> - Cellular Senescence Detection Kit - Lysosomal pH and mass detection Kit More about Lysosomal Function Analysis