Anti-Senescence Effects Induced by Extracellular Vesicles DOJINDO LABORATORIES

Previous Science Note

Anti-senescence study aims to restore youthful function to aging tissues and organs, often focusing on cellular repair and regeneration. Extracellular vesicles (EVs), including exosomes and microvesicles, play a critical role in cell-to-cell communication by transporting bioactive molecules such as proteins, lipids and RNA. Recent studies suggest that EVs derived from young or healthy cells can have anti-senescence effects on aged cells and tissues, promoting regeneration and repair. These EVs have shown potential to reverse age-related damage and improve tissue function, making them a promising tool in regenerative medicine and anti-aging therapies.
Small extracellular vesicles from young plasma reverse age-related functional declines by improving mitochondrial energy metabolism Click here for the original article: Xiaorui Chen, et. al., Nature Aging, 2024.	Extracellular vesicles from human urine-derived stem cells delay aging through the transfer of PLAU and TIMP1 Click here for the original article: Shanshan Rao, et. al., Acta Biomaterialia, 2024.	Rejuvenating effects of young extracellular vesicles in aged rats and in cellular models of human senescence Click here for the original article: Lilian Grigorian Shamagian, et. al., EMBP Reports, 2023.
Point of Interest - Young sEVs rejuvenate aged mice tissues and extend lifespan. - Proteomic analysis shows young sEVs enhance metabolism and mitochondrial function in aged tissues. - Young sEVs stimulate PGC-1α, reversing age-related dysfunction.	Point of Interest - Intravenous injection of human urine-derived stem cell EVs (USC-EVs) inhibit cellular senescence, improving cognitive and physical functions in mice. - USC-EVs' anti-aging effects are consistent regardless of donor age, gender, or health. - Plasminogen activator urokinase (PLAU) and tissue inhibitor of metalloproteinases 1 (TIMP1) in USC-EVs contribute to the anti-senescent effects of USC-EVs.	Point of Interest - Young cardiosphere-derived cells (CDC) EVs rejuvenate aged rats, improving heart, lungs, muscles, and kidneys. - Purified EVs from young blood show rejuvenating effects; EV-depleted serum increases senescence. - The treatment also favorably modulates glucose metabolism and anti-senescence pathways, ultimately extending lifespan.
Related Techniques
Cellular senescence detection	SPiDER-βGal for live-cell imaging or flow cytometry / microplate reader / tissue samples.
Exosome Labeling	ExoSparkler Exosome Membrane Labeling Kit - Green / Red / Deep Red
Exosome purification	ExoIsolator Exosome Isolation Kit, ExoIsolator Isolation Filter
Glycolysis/Oxidative phosphorylation Assay	Glycolysis/OXPHOS Assay Kit
Oxygen Consumption Rate(OCR) Plate Assay	Extracellular OCR Plate Assay Kit
Mitochondrial membrane potential detection	JC-1 MitoMP Detection Kit, MT-1 MitoMP Detection Kit
Mitochondrial superoxide detection	MitoBright ROS Deep Red - Mitochondrial Superoxide Detection
Total ROS detection	Highly sensitive DCFH-DA or Photo-oxidation Resistant DCFH-DA
Endocytosis Detection detection	ECGreen-Endocytosis Detection
Lysosomal function	Lysosomal Acidic pH Detection Kit-Green/Red and Green/Deep Red
Related Applications
Metabolic shift to glycolysis in senescenct cells
		NAD(+) levels decline during the aging process, causing defects in nuclear and mitochondrial functions and resulting in many age-associated pathologies. Here, we try to redemonstrate this phenomenon in the doxorubicin (DOX)-induced cellular senescence model with a comprehensive analysis of our products. S. Imai, et al., Trends Cell Biol, 2014, 24, 464-471 Products in Use ① DNA Damage Detection Kit - γH2AX ② Cellular Senescence Detection Kit - SPiDER-βGal ③ NAD/NADH Assay Kit-WST ④ JC-1 MitoMP Detection Kit ⑤ Glycolysis/OXPHOS Assay Kit、Lactate Assay Kit-WST