06 -Bacstain- Series

-Bacstain- DAPI solution

-<i>Bacstain</i>- DAPI solution

Bacterial Staining

  • Fluorescence detection
  • Ready-to-use solution
  • Product code
    BS04  -Bacstain- DAPI solution
  • CAS No.
    28718-90-3(DAPI)
  • Chemical name
    4',6-Diamidino-2-phenylindole, dihydrOchloride, solution
  • MW
    C16H17Cl2N5=350.25
Unit size Price Item Code
100 assays $104.00 BS04-10

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Chemical Structure

Manual

Technical info

1. Allow DAPI solution a) to stand at room temperature for 30 minutes. Solution should be protected from light.
2. Resuspend the organisms with PBS(-) or saline and adjust the number of cells to 106cells/mL(flow cytometry) or 108-109cells/mL(microscopy).
3. Add 1 μL of DAPI solution into the microbial cell suspension and vortex gently to mix. Formaldehyde-fixation can be recommended if necessary.
4. Incubate the microbial cells at room temperature for 5 minutes.
5. Analyze the stained-cells by a flow cytometer or a microscope. The maximum wavelengths of the dye are 360 nm for excitation and 460 nm for emission.

a) Since DAPI may be carcinogenic, be careful when handling and disposing.

Staining Data

L. casei stained with CTC and DAPI (left). B. cereus stained with CFDA and DAPI (right).

Handling and storage condition

Specification
Appearance: Colorless to yellow liquid
Dye content: To pass test
Handling and storage condition
0-5°C, Protect from light
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Product Classification

Product Classification

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