Observe Bacteria from Various Parameters

Observe Bacteria from Various Parameters


  • Prepare protocol optimized for fluorescence double staining
  • Evaluate effect of drug or bacterial conditions using multiple indicators
  • Drastically reduce evaluation time

Recommended for People who would like to:

  • Start fluorescent staining
  • Detect damaged or viable but non-culturable bacteria
  • Accurately and easily evaluate effect of drug to
Product Size Product Code
-Bacstain- Bacterial Viability Detection Kit-DAPI/PI 1 set BS08
-Bacstain- Bacterial Viability Detection Kit-CTC/DAPI 1 set BS09
-Bacstain- Bacterial Viability Detection Kit-CFDA/PI 1 set BS10

Protocol optimized for double staining

Select parameter for your experiment

Correlation map of related indicators

-Bacstain- Bacterial Viability Detection Kit series contain all reagents which are required for double staining and allows to observe bacterial conditions using multiple parameters.

Why should we use multiple parameters ?

In the evaluation of effect of antibacterial agents, colony forming unit quantification or multiple staining method with fluorescent dyes are widely used. In recent years, issues such as the appearance of drug-resistant bacteria in infection diseases or food poisoning lead by damaged bacteria are reported. Requirement of antibacterial evaluation using multiple parameters has an increased need to improve the reliability in antibacterial evaluation.

Experimental example

Evaluate Bacteria from Various Parameters

-Bacstain- Bacterial Viability Detection Kit series were utilized to stain staphylococcus aureus.

Quantification using image analysis tool

-Bacstain- Bacterial Viability Detection Kit series were utilized to evaluate the effect of benzalkonium chloride to S. aureus (Gram-positive). The acquired staining image was quantified by image analysis tool and the correlation with the concentration of drug was shown. The different effect of benzalkonium chloride between respiration activity and membrane damage was confimed.

Product Classification

Product Classification