-Bacstain- DAPI solutionBS04 Product Manual

-Bacstain- series offer several kinds of bacterial fluorescence staining dye and can be applied for microbial cell viability assay in different principles.
DAPI is an AT-sequence specific DNA intercalator that attaches to DNA at the minor groove of the double helix.
-Bacstain-DAPI solution is a Ready-to-Use solution.

DAPI aqueous solution

25 μl x 4, 1 mg/ml

Store at 0-5°C.

• Fluorescence microscope (ultraviolet excitation light, blue emission filter) or Flow cytometer (ultraviolet light, blue emission filter)
• Micropipette (20 μl, 1,000 μl)

This kit includes microtubes containing solutions. Since there is a possibility that the droplets might attach to the inside walls or caps, please shake them down prior to open.

1. Allow DAPI solution to stand at room temperature for 30 minutes. Solution should be protected from light ^a).
2. Resuspend the organisms with PBS(-) or saline and adjust the number of cells to 10⁶ cells/ml (flow cytometry) or 10⁸ -10⁹ cells/ml (microscopy).
3. Add 1μl of DAPI solution into the 1 ml of microbial cell suspension and vortex gently to mix.
4. Incubate the microbial cells at room temperature for 5 minutes.
5. Analyze the stained cells by flow cytometer or under a microscope.

 

a) Since DAPI may be carcinogenic, please be careful in its handling/disposing.

This kit can perform at least 100 tests for the flow cytometric assay and microscopic assay by following the protocol described herein.

If -Bacstain- CTC Rapid Staining Kit or -Bacstain- CFDA solution is used with DAPI, double-staining examination can be performed.

-Bacstain- CTC Rapid Staining Kit (for Flow cytometry)

-Bacstain- CTC Rapid Staining Kit (for Microscopy)

CTC has been used by many researchers to evaluate the microbial respiratory activity.
-Bacstain- CTC Rapid Staining Kit allows quick and high-sensitivity CTC-staining.

CTC staining efficiencies were compared in with or without enhancing reagent condition.

-Bacstain- CFDA solution

CFDA is widely used as an indicator for the measurement of esterase activity.
-Bacstain- CFDA solution is provided as Ready-to-Use DMSO solution.
Fluorescent carboxyfluorescein is produced from non fluorescent CFDA by the esterase in the microbial cell.

 

-Bacstain- AO solution

AO is nucleic acid staining dyes and can be applied for bacteria either with membrane impairment or with membrane intact.

 

-Bacstain- PI solution

PI is a nucleic acid staining dye. Membrane-injured cells are stained with red emission by PI.

 

Products	Code	Maximum Ex/Em(nm)	Number of assays
CTC Rapid Staining Kit (for Flow cytometry)	BS01	430, 480/630	100
CTC Rapid Staining Kit (for Microscopy)	BS02	430, 480/630	100
CFDA solution	BS03	493/515	100
DAPI solution	BS04	360/460	100
AO solution	BS05	420-460/630-650(ssDNA)	100
		500/520(dsDNA)
PI solution	BS07	530/620	100