Aerobic Glycolysis Impact on Tumor's Sensitivity to T-Cell Killing DOJINDO LABORATORIES

Previous Science Note

Scientists have found that deficiency of two key glycolytic enzymes, Glut1 (glucose transporter 1) and Gpi1 (glucose-6-phosphate isomerase 1), enhanced tumor cell killing by cytotoxic T cells. Genetic and pharmacologic inactivation of Glut1 sensitizes tumors to anti-tumor immunity and synergizes with anti-PD-1 therapy through the TNF-α pathway.

Tumor aerobic glycolysis confers immune evasion through modulating sensitivity to T cell-mediated bystander killing via TNF-α
Click here for the original article: Lijian Wu, et. al., Cell Metabolism, 2023.

Point of Interest
- The intrinsic glycolysis pathway within tumors confers resistance to killing by T cells.

- Depletion of Glut1 sensitizes tumor cells to cell death induced by TNF-α through the augmentation of reactive oxygen species (ROS).

- Glut1 and Glut3 exhibit differential expression in tumor cells compared to immune cells.

- Inhibiting Glut1 enhances the anti-tumor immune response in murine models.

Related Techniques

Glycolysis/Oxidative phosphorylation assay

Glycolysis/OXPHOS Assay Kit

Nutrient uptake Assay

Glucose (Blue / Green / Red), Amino Acid, Cystine, and Fatty Acid Uptake Assay Kit

Glycolysis-related metabolites assay

Glucose and Lactate Assay Kit

TCA cycle-related metabolites assay

Glutamine, Glutamate, and α-KG Assay Kit

NAD(H) and NADP(H) redox couples assay

NAD/NADH and NADP/NADPH Assay Kit

Mitochondrial function/glycolysis detection

Glycolysis/JC-1 MitoMP Assay Kit

Oxygen consumption rate assay

Extracellular OCR Plate Assay Kit

Related Applications

Metabolic shift to glycolysis in senescenct cells

＜Evaluation by Lactate production and ATP levels＞

NAD(+) levels decline during the aging process, causing defects in nuclear and mitochondrial functions and resulting in many age-associated pathologies*. Here, we try to redemonstrate this phenomenon in the doxorubicin (DOX)-induced cellular senescence model with a comprehensive analysis of our products.

*S. Imai, et al., Trends Cell Biol, 2014, 24, 464-471

Products in Use
① DNA Damage Detection Kit - γH2AX
② Cellular Senescence Detection Kit - SPiDER-βGal
③ NAD/NADH Assay Kit-WST
④ JC-1 MitoMP Detection Kit
⑤ Glycolysis/OXPHOS Assay Kit、Lactate Assay Kit-WST