Science Note
[July 11, 2023] Previous Science Note
Lysosomal pH regulated by LAMP proteins
Through a combination of various scientific methods, this research reveals the vital role of human lysosome-associated membrane proteins (LAMP-1 and LAMP-2) in maintaining lysosomal pH balance. Despite being mainly recognized as lysosomal markers, LAMP-1 and LAMP-2 directly influence the lysosomal cation channel TMEM175, aiding the acidification of lysosomes to a pH level that optimizes hydrolase activity. Interruptions to the interaction between LAMP and TMEM175 can increase lysosomal pH, impairing their hydrolytic function. |
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Lysosomal LAMP proteins regulate lysosomal pH by direct inhibition of the TMEM175 channel Point of Interest |
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Related Techniques | |
Lipid droplets detection | Lysosomal Acidic pH Detection Kit-Green/Red, Green/Deep Red |
Lysosome staining | pH-dependent (Red) and pH-independent (Green / Deep Red) probes |
Autophagy detection | DAPGreen / DAPRed (Autophagosome detection), DALGreen (Autolysosome detection) |
Endocytosis detection | ECGreen |
Endocytic internalization assay | AcidSensor Labeling Kit |
Plasma membrane staining | PlasMem Bright Green / Red |
Total ROS detection | Highly sensitive DCFH-DA or Photo-oxidation Resistant DCFH-DA |
Related Applications | |
Accurate Measurement for Lysosomal pH changesExisting lysosomal pH detection reagents have issues with dye localization, pH sensitivity, and retention. pHLys Green is a dye that solves these issues. The improved dye retention and localization enable detection of normal lysosomes, and the improved pH sensitivity enables detection of slight pH changes.
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