[July 11, 2023] Previous Science Note
Lysosomal pH regulated by LAMP proteins
Through a combination of various scientific methods, this research reveals the vital role of human lysosome-associated membrane proteins (LAMP-1 and LAMP-2) in maintaining lysosomal pH balance. Despite being mainly recognized as lysosomal markers, LAMP-1 and LAMP-2 directly influence the lysosomal cation channel TMEM175, aiding the acidification of lysosomes to a pH level that optimizes hydrolase activity. Interruptions to the interaction between LAMP and TMEM175 can increase lysosomal pH, impairing their hydrolytic function.
Point of Interest
|Lipid droplets detection||Lysosomal Acidic pH Detection Kit-Green/Red, Green/Deep Red|
|Lysosome staining||pH-dependent (Red) and pH-independent (Green / Deep Red) probes|
|Autophagy detection||DAPGreen / DAPRed (Autophagosome detection), DALGreen (Autolysosome detection)|
|Endocytic internalization assay||AcidSensor Labeling Kit|
| Plasma membrane staining||PlasMem Bright Green / Red|
|Total ROS detection||Highly sensitive DCFH-DA or Photo-oxidation Resistant DCFH-DA|
Accurate Measurement for Lysosomal pH changes
Existing lysosomal pH detection reagents have issues with dye localization, pH sensitivity, and retention. pHLys Green is a dye that solves these issues. The improved dye retention and localization enable detection of normal lysosomes, and the improved pH sensitivity enables detection of slight pH changes.
Product in Use: