20 Functional Organic Material

Biotin-SAM Formation Reagent

Biotin-SAM Formation Reagent

Self Assembled Monolayer Reagent

  • Simple preparation of Biotin-SAM on gold surface
  • Efficient Immobilization of Avidins
  • Suppress unspecific binding with unknown proteins
  • Product code
    B564  Biotin-SAM Formation Reagent
Unit size Price Item Code
1 umol x 3 $245.00 B564-10
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1 umol x 3
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Product Description

There are three ways to immobilize a protein on a Surface Plasmon Resonance (SPR) or Quartz Crystal Microbalance (QCM) biosensor consisting of Self-Assembled Monolayer (SAM); 1) Formation of covalent bond of amine functions on proteins and activated carboxy-SAM; 2) immobilize proteins by His-Tag through Ni-NTA-terminated SAM; 3) Immobilization of proteins through Biotin and Avidin interaction. Because the Biotin-Avidin formation is known as a facile reaction, it is widely utilized for immobilization of protein (Fig 1).

Fig.1 A biosensor construction by Biotin-SAM Formation Reagent

 

Fig. 2 Comparison of the amount of bound streptavidin, unspecific binding of FBS, bound biotinylated BSA between Biotin-SAM Formation Reagent and Company A.

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Handling and storage condition
0-5°C
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