20 Functional Organic Material

Amine Coupling Kit

Amine Coupling Kit

Self Assembled Monolayer Kit

  • Immobilization of protein/peptides onto SAMs with terminal carboxylic acid
  • Including all necessary reagents for activation, immobilization, and blocking
  • Sufficient for 40 immobilizations
  • Product code
    A515  Amine Coupling Kit
Unit size Price Item Code
2 ml x 4 $300.00 A515-10
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Component
2 ml x 4 [2ml x 4]
WSC
NHS
Activation Buffer
Reaction Buffer
Blocking Solution

4 tubes
4 tubes
20 ml x 1
10 ml x 1
20 ml x 1

Description

Amine and carboxylic acid coupling is one of the most common methods to immobilize a protein or a peptide through a covalent bound onto biosensor surface. Dojindo’s Amine Coupling Kit contains all the reagents and buffers necessary reagents and buffers for activation of carboxylic acid, protein immobilization, and blocking. Blocking solution included in the kit minimizes non-specific protein absorption to the surface by capping residual activated esters. Each kit is adequate for approximately 40 immobilizations.

Technical info

Example of Solvents

100 mmol/l (NHS solution): Dissolve NHS 1 tube in Activation Buffer 1 ml in the kit
100 mmol/l (WSC solution): Dissolve WSC 1 tube in Activation Buffer 1 ml in the kit

References

Open References

Expression and assembly of active human cardiac troponin in Escherichia coli MW Lassalle - Protein expression and purification, 2012

Handling and storage condition

Handling and storage condition
0-5°C
Danger / harmful
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Product Classification

Product Classification

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