Diverse Functions of Senescent Cells DOJINDO LABORATORIES

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Cellular senescence, which contributes significantly to aging, is controlled by various factors such as cell type and physiological conditions, such as oxidative stress. It has been reported that these senescent cells have various negative effects, and treatment methods aimed at removing these senescent cells (Senotherapeutics) have been attracting attention. On the other hand, there are also reports of positive effects, and more detailed studies on the functions of senescent cells will be needed in the future. Today, we introduce you to three highlighted articles related to cellular senescence focusing on Senotherapeutics, Positive and negative impacts on tissue regeneration.
Senotherapeutics in Alzheimer's disease	Negative impacts on tissue regeneration	Positive impacts on tissue regeneration
Increased post-mitotic senescence in aged human neurons is a pathological feature of Alzheimer’s disease (Joseph R. Herdy, et al., Cell Stem Cell, 29, 1637-1652, 2022)	Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration (Victoria Moiseeva, et al., Nature, 613, 169-178, 2023)	Sentinel p16INK4a+ cells in the basement membrane form a reparative niche in the lung (Nabora S. Reyes, et al., Science, 378, 192-201, 2022)
- Aged, induced neurons (iNs) endogenously present features of cellular senescence - Alzheimer’s neurons senesce more frequently than healthy controls - Senescent neurons gain an inflammatory senescence-associated secretory phenotype - Senescent neurons can be eliminated with senotherapeutics	- The authors developed a method for isolating senescent cells from damaged muscles in mice - There are different senescent cell types from damaged muscles of young and old mice - These senescent cells arrest stem cell proliferation and regeneration by creating an inflamed niche. - By reducing senescent cells or neutralizing their inflammatory secretions, tissue regeneration was accreted in young and old mice.	- A new reporter has been created to detect p16INK4a, a biomarker of cellular senescence. - p16INK4a-expressing fibroblasts with certain senescent characteristics appeared near epithelial stem cells in the lung shortly after birth. - These p16INK4a+ fibroblasts have a heightened ability to sense tissue inflammation and promote epithelial regeneration. - p16INK4a expression is necessary for fibroblasts to enhance epithelial regeneration.
Related Technique in This Topic
Cellular senescence detection (Live cell imaging or FCM)	Cellular Senescence Detection Kit - SPiDER-βGal
Cellular senescence detection (Plate reader)	Cellular Senescence Plate Assay Kit - SPiDER-βGal HOT
Oxygen consumption rate assay	Extracellular OCR Plate Assay Kit NEW
Glycolysis/Oxidative phosphorylation Assay	Glycolysis/OXPHOS Assay Kit HOT
NAD/NADH assay	NAD/NADH Assay Kit
Mitochondrial membrane potential detection	JC-1 MitoMP Detection Kit and MT-1 MitoMP Detection Kit
Mitochondrial superoxide detection	MitoBright ROS Deep Red - Mitochondrial Superoxide Detection HOT
Total ROS detection	Higher sensitivity HOT or compatible with co-staining for immunostaining NEW