Mitochondrial unfolded protein response in neural stem cell aging DOJINDO LABORATORIES

Previous Science Note

This article focusing on the mitochondrial protein folding stress is particularly significant in activated neural stem cells (NSCs) and neural progenitor cells (NPCs) within the neurogenic niche. This stress increases with age, leading to dysregulated cell cycles and mitochondrial activity in activated NSCs and NPCs in the dentate gyrus.

The mitochondrial unfolded protein response regulates hippocampal neural stem cell aging

Click here for the original article: Chin-Ling Wang, et. al., Cell Metabolism (2023)

Point of Interest

- Elevated mitochondrial protein folding stress in aging neural stem cells
- SIRT7 safeguards neural stem cells by reducing mitochondrial protein folding stress
- SIRT7 overexpression enhances neurogenesis and cognitive function in aged mice

Related Techniques

Glycolysis/Oxidative phosphorylation assay

Glycolysis/OXPHOS Assay Kit

Oxygen consumption rate assay

Extracellular OCR Plate Assay Kit

Mitochondrial membrane potential detection

JC-1 MitoMP Detection Kit / MT-1 MitoMP Detection Kit

Mitochondrial superoxide detection

MitoBright ROS - Mitochondrial Superoxide Detection

Mitophagy Detection

Mitophagy Detection Kit and Mtphagy Dye

Cellular senescence detection (Live cell imaging or FCM)

Cellular Senescence Detection Kit

Cellular senescence detection (Plate reader)

Cellular Senescence Plate Assay Kit

Lysosomal function assay

Lysosomal pH and mass detection Kit

Related Applications

Metabolic shift to glycolysis in senescenct cells

NAD(+) levels decline during the aging process, causing defects in nuclear and mitochondrial functions and resulting in many age-associated pathologies*. Here, we try to redemonstrate this phenomenon in the doxorubicin (DOX)-induced cellular senescence model with a comprehensive analysis of our products.

*S. Imai, et al., Trends Cell Biol, 2014, 24, 464-471

Products in Use
① DNA Damage Detection Kit - γH2AX
② Cellular Senescence Detection Kit - SPiDER-βGal
③ NAD/NADH Assay Kit-WST
④ JC-1 MitoMP Detection Kit
⑤ Glycolysis/OXPHOS Assay Kit、Lactate Assay Kit-WST

Lysosomal Function and Mitochondrial ROS

CCCP and Antimycin are recognized inducers of mitochondrial ROS, linked to the loss of mitochondrial membrane potential. Recent studies have shown that CCCP induces not only mitochondrial ROS but also lysosomal dysfunction. To observe mitochondrial ROS, HeLa cells were labeled with MitoBright ROS Deep Red for Mitochondrial Superoxide Detection, and the lysosomal mass and pH were independently detected with LysoPrime Green and pHLys Red. Co-staining with MitoBright ROS and Lysosomal dyes revealed that CCCP, unlike Antimycin, triggers concurrent lysosomal neutralization and mitochondrial ROS induction.

Reference: Benjamin S Padman, et. al., Autophagy (2013)

Products in Use
- LysoPrime Green
- pHLys Red
- Lysosomal Acidic pH Detection Kit
- MitoBright ROS - Mitochondrial Superoxide Detection