Science Note
[May 9, 2023] Previous Science Note
Link Lysosomal Failure to Ferroptosis in Human Neurons
Here, the scientists reveal an unexpected role for the lysosomal protein prosaposin (PSAP), the knockdown of which caused the formation of lipofuscin, a hallmark of aging, which traps iron, generating reactive oxygen species and triggering ferroptosis. Intriguingly, PSAP deficiency caused these dramatic phenotypes only in neurons, but not in other cells. Learn how the authors used Dojindo's Ferroptosis-related products, FerroOrange and Liperfluo for detecting Iron levels and lipid peroxidation, in their study. | |||||
Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis Ruilin Tian, et. al., Nature Neuroscience (2022) Point of Interest |
|||||
Related Techniques | |||||
Intracellular lipid peroxidation measurement | Liperfluo | ||||
Mitochondria lipid peroxidation measurement | MitoPeDPP | ||||
Intracellular ferrous ion (Fe2+) detection | FerroOrange | ||||
Mitochondria ferrous ion (Fe2+) detection | Mito-FerroGreen | ||||
Mitochondrial superoxide detection | mtSOX Deep red - Mitochondrial superoxide Detection | ||||
Lysosomal function assay | Lysosomal pH and mass detection Kit | ||||
Cellular senescence detection (Live cell imaging or FCM) | Cellular Senescence Detection Kit | ||||
Cellular senescence detection (Plate reader) | Cellular Senescence Plate Assay Kit | ||||
Related Applications | |||||
The simultaneous detection of lysosomal function with Mitochondrial ROS and intracellular Fe2+
|
|||||
Induction of Ferroptosis by ErastinErastin is a known inducer of ferroptosis. By inhibiting the cystine transporter (xCT), erastin inhibits the uptake of cystine. Cystine is the raw material for GSH. Therefore, Erastin ultimately decreases the amount of GSH. Decreased GSH then results in lipid peroxide accumulation and induction of ferroptosis. Using erastin-treated A549 cells, we measured intracellular Fe2+, ROS, lipid peroxide, glutathione, glutamate release into the extracellular space, and cystine uptake. As a result, inhibition of xCT by elastin was observed and also the release of glutamate and uptake of cystine were decreased. Furthermore, elastin treatment decreased intracellular glutathione while it increased intracellular Fe2+ , ROS, and lipid peroxides.
|