Science Note
[July 25, 2023] Previous Science Note
Ferroptosis Induced by FSP1-dependent Phase Separation
Ferroptosis suppressor protein-1 (FSP1) has recently been identified as a second system that suppresses ferroptosis, preventing lipid peroxidation independently of the glutathione-GPX4 axis. Researchers have used a small molecule library screen to discover 3-phenylquinazolinones, represented by icFSP1, as potent inhibitors of FSP1. Unlike previous inhibitors, icFSP1 does not directly inhibit FSP1 enzyme activity, but causes FSP1 to relocate from the membrane and condense, working in synergy with GPX4 inhibition. Learn more about how the authors detected Lipid peroxide, one of the ferroptosis markers, using Liperfluo. We also offer ferroptosis-related products: FerroOrange, Mito-FerroGreen. |
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Phase separation of FSP1 promotes ferroptosis Click here for the original article: T. Nakamura, et. al., Nature (2023) Point of Interest |
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Related Techniques | |||||||||||||||||||
Intracellular lipid peroxidation measurement | Liperfluo | ||||||||||||||||||
Mitochondria lipid peroxidation measurement | MitoPeDPP | ||||||||||||||||||
Intracellular ferrous ion (Fe2+) detection | FerroOrange | ||||||||||||||||||
Mitochondria ferrous ion (Fe2+) detection | Mito-FerroGreen | ||||||||||||||||||
Mitochondrial superoxide detection | mtSOX Deep red - Mitochondrial superoxide Detection | ||||||||||||||||||
Total ROS detection | Highly sensitive DCFH-DA or Photo-oxidation Resistant DCFH-DA | ||||||||||||||||||
Lipid droplets detection | Lipi-Blue / Green / Red / Deep Red | ||||||||||||||||||
NAD(H) and NADP(H) redox couples assay | NAD/NADH and NADP/NADPH Assay Kit | ||||||||||||||||||
Related Applications | |||||||||||||||||||
Erastin-Induced Ferroptosis: Evaluating Intracellular Uptake and Redox Balance
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