01 Cell Proliferation / Cell Cytotoxicity Assay

Viability/Cytotoxicity Multiplex Assay Kit

Cell Proliferation / Cell Cytotoxicity Assay

Cell Proliferation / Cytotoxicity Assay Kit

Set of Cell Counting Kit-8 and Cytotoxicity LDH Assay Kit-WST (500 tests each)

Product code

CK17　 Viability/Cytotoxicity Multiplex Assay Kit

Unit size	Price	Item Code
500 tests		CK17-10

Component

500 tests	Cell Counting Kit-8 [500 tests] ・WST-8, 1-Methoxy PMS Cytotoxicity LDH Assay Kit-WST [500 tests] ・Dye Mixture　　　　　・Assay Buffer ・Lysis Buffer ・Stop Solution	5ml x1 x1 55 ml x1 5.5 ml x1 27.5 ml x1

C of A

SDS download

Product Description

In order to confirm cytotoxicity, evaluation using only live cells or only dead cells as indicators may not be sufficiently reliable, and therefore, there are an increasing number of cases where experiments are supported by evaluating multiple indicators based on different measurement principles.

This kit is a set of two different indicators, Cell Counting Kit-8 (CCK-8) and Cytotoxicity LDH Assay Kit-WST (LDH Assay Kit). An assay manual is available to evaluate these two assays using the same sample.

Manual

Manual English

Technical info

When confirming cytotoxicity, evaluation using only live cells or only dead cells as indicators may not be sufficiently reliable, and therefore, more and more experiments are being supported by evaluation using multiple indicators based on different measurement principles.

Experimental Example

Cell Counting Kit-8 and Cytotoxicity LDH Assay Kit-WST measure dehydrogenase activity and released amount of LDH as reflective of living cells and dead cells, respectively. According to the results with anticancer drug, the number of living cells was reduced, and the number of dead cells increased as the concentration of Mitomycin C increased. Interestingly, a low concentration of Mitomycin C (green frame) inhibits cell proliferation earlier than inducing cell death unlike a high concentration (red frame).

Measuring Principle

LDH Assay Kit: The activity of LDH leaked out of cells due to cell membrane damage can be confirmed as an indicator of dead cells.
Cell Counting Kit-8: Metabolic activity in living cells can be confirmed using NADH as an indicator.

The same cell sample can be applied

This kit includes CCK-8 for measuring live cells and LDH Assay Kit for measuring dead cells, both of which can be evaluated by plate assay at the same absorbance wavelength. The same cell sample can be used to evaluate both live and dead cells at the same time, saving time and labor.

References evaluating cytotoxicity using CCK-8 and the LDHAssay Kit.

Cell Type	Smaple	Title of reference	Reference
C3H10T1/2	Pirfenidone	XL413, a cell division cycle 7 kinase inhibitor enhanced the anti-fibrotic effect of pirfenidone on TGF-s1-stimulated C3H10T1/2 cells via Smad2/4）	S. Jin, et. al, Exp Cell Res., 2015, 339(2), 289-99
Human liver cell line L02	S17092 (POP inhibitor)	Prolyl Oligopeptidase Inhibition Attenuates Steatosis in the L02 Human Liver Cell Line	Zhou D, et al., PLOS ONE, 2016, 11(10)
RAW264.7	FAC (Ferric ammonium citrate) IFN-gamma	Iron Reduces M1 Macrophage Polarization in RAW264.7 Macrophages Associated with Inhibition of STAT1	Zhen-Shun Gan, et. al, Mediators of Inflammation, 2017
Mouse embryonic Fibroblast (MEF)	Methylmercury (MeHg)	Atg5-dependent autophagy plays a protective role against methylmercury-induced cytotoxicity	Takanezawa Yasukazu, et. al, Toxicity Letters, 2016, 262, 135-141