09 Cross-Linking Reagents

AB-NTA free acid

AB-NTA free acid

Chelate Labeling Reagent

  • Product code
    A459  AB-NTA free acid
  • CAS No.
    129179-17-5
  • Chemical name
    N-(5-Amino-1-carboxypentyl)iminodiacetic acid
  • MW
    C10H18N2O6=262.26
Unit size Price Item Code
100 mg $170.00 A459-10
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Description

Product Description

Aminobutyl-NTA (AB-NTA) free acid replaces AB-NTA (disodium salt form; former Product Code: A296-10). The new free acid form is less hygroscopic than the disodium salt form. Dissolving the new acid form in a buffer involves the same procedure as dissolving disodium salt in a buffer. The new acid form can also be dissolved in water with sonication (up to 3%). Aminobutyl-NTA was first used by Dr. Hochuli in 1987 to purify recombinant proteins (the “Histag Etechnique). Since then, the AB=NTA has become an indispensable tool for immobilizing proteins with high specificity on solid surfaces, such as glass or the gold electrode. A solid surface is modified by AB-NTA and bio-functionalized via Ni (II) with a genetically expressed protein bearing a hexahistidine extension at its terminus. The His-tag technique has become increasingly important, particularl in surface plasmon resonance and structural analyses of proteins by x-ray interference. Using the His-tag technique, Dr. Noji was able to directly observe the rotation of F1-ATPase by fluorescence microscope.

 

Chemical Structure

References

Open References

1. E. Hochuli, H. Doeli and A. Schacher, New Metal Chelate Adsorbent Selective for Proteins and Peptides Containing Neighbouring Histidine Residues, J. Chromatogr., 1987, 411, 177.
2. E. Hochuli, Large-scale Chromatography of Recombinant Proteins, J. Chromatogr., 1988, 444, 293.
3. Y. C. Sasaki, Y. Suzuki and T. Ishibashi, Fluorescent X-ray Interference from a Protein Monolayer, Science, 1994, 263, 62.
4. G. B. Sigal, C. Bamdad, A. Barberis, J. Strominger and G. M. Whitesides, A Self-assembled Monolayer for the Binding and Study of Histidine-tagged Proteins by Surface Plasmon Resonance, Anal. Chem., 1996, 68, 490.
5. E. L. Schmid, T. A. Keller, Z. Dienes and H. Vogel, Reversible Oriented Surface Immobilization of Functional Proteins on Oxide Surface, Anal. Chem., 1997, 69, 1979.
6. R. Yasuda, H. Noji, K. Kinosita, and M. Yoshida, F1-ATPase is a Highly Efficient Molecular Motor that Rotates with Discrete 120°Steps, Cell, 1998, 93, 1117.

Handling and storage condition

Specification
Appearance: White to pale yellowish white powder
Purity (HPLC): ≧ 97.0 %
Solubility in water: To pass test (clear, colorless to slightly yellow)
IR spectrum: Authentic
NMR spectrum: Authentic
Handling and storage condition
Ambient temperature
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Product Classification

Product Classification

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