09 Cross-Linking Reagents




  • Product code
    M035  Maleimido-C3-NTA
  • CAS No.
  • Chemical name
    N-[5-(3'-Maleimidopropylamido)-1-carboxypentyl]iminodiacetic acid, disodium salt, monohydrate
  • MW
Unit size Price Item Code
10 mg $242.00 M035-10
10 mg
Quantity may be adjusted within the cart.

Product Description

Maleimido-C3-NTA is utilized to modify the surfaces on which thiol groups are attached. Through the NTA moiety attached to the surface, genetically expressed protein, bearing a hexahistidine extension at its terminus, can be immobilized via Ni (II) (His-Tag method). Using this technique, Dr. Noji and co-workers were able to directly observe the rotation of F1-ATPase with a fluorescence microscope.

Chemical Structure


Open References

1) E. Hochuli, H. Doeli and A. Schacher, "New Metal Chelate Adsorbent Selective for Proteins and Peptides Containing Neighbouring Histidine Residues", J. Chromatogr.1987411, 177.
2) E. Hochuli, "Large-Scale Chromatography of Recombinant Proteins", J. Chromatogr.1988444, 293.
3) Y. C. Sasaki, Y. Suzuki and T. Ishibashi, "Fluorescent X-ray Interference from a Protein Monolayer", Science1994263, 62.
4) G. B. Sigal, C. Bamdad, A. Barberis, J. Strominger and G. M. Whitesides, "A Self-Assembled Monolayer for The Binding and Study of Histidine-Tagged Proteins by Surface Plasmon Resonance, Anal. Chem.1996, 68, 490.
5) E. L. Schmid, T. A. Keller, Z. Dienes and H. Vogel, "Reversible Oriented Surface Immobilization of Functional Proteins on Oxide Surface", Anal. Chem., 199769, 1979.

Handling and storage condition

Appearance: White pale yellowish-white powder
Purity (HPLC): ≥97.0% (HPLC)
Solubility in water: To pass test (clear, colorless)
IR spectrum: Authentic
NMR spectrum: Authentic
Handling and storage condition
0-5°C, Protect from moisture

Product Classification

Product Classification

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