19 Biochemicals



Condensation Reagent for Peptide Synthesis

  • Product code
    W001  WSC
  • CAS No.
  • Chemical name
    1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide, hydrOchloride
  • MW
Unit size Price Item Code
5 g $85.00 W001-10
25 g $289.00 W001-12
Quantity may be adjusted within the cart.


Chemical Structure


Product Description of Calixarenes
WSC is a water soluble carbodiimide. It is also soluble in alcohol, acetone, chloroform, dioxane, and DMF. WSC is used as a condensing agent for peptide syntheses. WSC and its urea form can be easily removed after the reaction due to their high water solubility. WSC, EDC, EDAC, EDCI, and ethyl-CDI are the same reagent.


Open References

1) J. H. Saunders and R. J. Slocombe, "The Chemistry of the Organic Isocyanates", Chem. Rev., 1948, 45, 203.
2) R. J. Slocombe, Edgar E. Hardy, J. H. Saunders and R. J. Jenkins, "Phosgene Derivatives. The Preparation of Isocyanates, Carbamyl Chlorides, and Cyanuric Acid", J. Am. Chem. Soc., 1950, 72, 1888.
3) K. D. Kopple and D. E. Nittecki, "Reactivity of Cyclic Peptides. II. Cyclo-L-tyrosyl-L-histidyl and Cyclo-L-tyrosyltriglycyl-L-histidylglycyl", Org. Biol. Chem., 1962, 84, 4457.
4) H. Ozawa, "Reaction of Insulin with Ethyl Glycinate and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide", Biochemistry, 1970, 9, 2158.
5) T. Y. Lin, "Lactose Synthetase. Modification of Carboxyl Groups in α-Lactalbumin", Biochemistry, 1970, 9, 984.
6) J. C. Sheehan and S. L. Ledis, "Total Synthesis of a Monocyclic Peptide LactoneAntibiotic, Etamycin", J. Am. Chem. Soc., 1973, 95, 875.
7) M. E. Addy, G. Steinman and M. F. Mallette, "A Problem in the Mechanism of Carbodiimide-Mediated Synthesis of Peptides in Aqueous Medium", Biochem. Biophys. Res. Commun., 1973, 52, 1034.
8) A. Williams and I. T. Ibrahim, "A New Mechanism Involving Cyclic Tautomers for the Reaction with Nucleophiles of the Water-Soluble Peptide Coupling Reagent 1-Ethyl-3-(3-(Dimethylamino)Propyl)Carbodiimide(EDC)", J. Am. Chem. Soc., 1981, 103, 7090.
9) H. Yamada, T. Imoto, K. Fujita, K. Okazaki and M. Motomura, "Selective Modification of Aspartic Acid-101 in Lysozyme by Carbodiimide Reaction", Biochemistry, 1981, 20, 4836.
10) J. W. Chase, B. M. Merrill, and K. R. Williams, "F Sex Factor Encodes a Single-Stranded DNA Binding Protein (SSB) with Extensive Sequence Homology to Escherichia Coli SSB", Proc. Natl. Acad. Sci. USA, 1983, 80, 5480.
11) J. V. Staros, R. W. Wright and D. M. Swingle, "Enhancement by N-Hydroxysulfosuccinimide of Water-Soluble Carbodiimide-Mediated Coupling Reactions", Anal. Biochem., 1986, 156, 220.
12) M. Taniuchi, H. B. Clark and E. M. Johnson Jr., "Induction of Never Growth Factor Receptor in Schwann Cells After Axotomy", Proc. Natl. Acad. Sci. USA, 1986, 83, 4094.
13) M. Taniuchi, J. B. Schweitzer and E. M. Johnson Jr., "Never Growth Factor Receptor Molecules in Rat Brain", Proc. Natl. Acad. Sci. USA, 1986, 83, 1950.

Handling and storage condition

Appearance: White powder
Purity (Titration): ≧ 98.0 %
Solubility in water: To pass test (clear, colorless)
Solubility in Methylene chloride: To pass test (clear, colorless)
m.p.: 113 - 121°C
IR spectrum: Authentic
Handling and storage condition
Protect from moisture

Product Classification

Product Classification

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