08 Labeling Chemistry
09 Cross-Linking Reagents

SPDP

SPDP

Cross-Linker

  • Product code
    S291  SPDP
  • CAS No.
    68181-17-9
  • Chemical name
    N-Succinimidyl 3-(2-pyridyldithio)propionate
  • MW
    C12H12N2O4S2=312.37
Unit size Price Item Code
100 mg $491.00 S291-10
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Description

Product Description
SPDP, a hetero-bifunctional reagent, is useful for bridging two different proteins, such as enzymes and antibodies. SPDP first reacts with a protein molecule through its amino groups. SPDP introduces a pyridyl disulfide moiety to the protein, which is then reduced by DTT to form thiol groups. These thiol groups then form a disulfide bond with another protein molecule thus yielding a heterodimer protein.

 

Structural Formula

Technical info

Homo-bifunctional Reagents

Product Name Code Length (Å) Solvent
DSP D629 8.5 DMSO, DMF
SPDP S291 4.1 Acetonitrile

References

Open References

1) P. Walden, Z. A. Nagy and J. Klein, "Major Histocompatibility Complex-Restricted and Unrestricted Activation of Helper T Cell Lines by Liposome-bound Antigens", J. Mol. Cell. Immunol19862, 191. 
2) R. D. Gordon, W. E. Fieles, D. L. Schotland, R. Hogue-Angeletti and R. L. Barchi, "Topographical Localization of the C-terminal Region of the Voltage-dependent Sodium Channel from Electrophorus Electricus Using Antibodies Raised Against a Synthetic Peptide", Proc. Natl. Acad. Sci. USA1987, 84, 308.

References

1. P. Walden, Z. A. Nagy and J. Klein, Major Histocompatibility Complex-Restricted and Unrestricted Activation of Helper T Cell Lines by Liposome-bound Antigens, J. Mol. Cell. Immunol, 1986, 2, 191.
2. R. D. Gordon, W. E. Fieles, D. L. Schotland, R. Hogue-Angeletti and R. L. Barchi, Topographical Localization of the C-terminal Region of the Voltagedependent Sodium Channel from Electrophorus Electricus Using Antibodies Raised Against a Synthetic Peptide, Proc. Natl. Acad. Sci. USA, 1987,84, 308.

Handling and storage condition

Specification
Appearance: White to slightly yellow powder
Purity (HPLC): ≧ 98.0 %
Solubility in acetonitrile: To pass test (clear, colorless to slightly yellow)
NMR spectrum: Authentic
Handling and storage condition
0-5°C
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Product Classification

Product Classification

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