09 Cross-Linking Reagents




  • Product code
    D630  DTSSP
  • CAS No.
    81069-02-5(free acid)
  • Chemical name
    Dithiobis(sulfosuccinimidyl propionate), disodium salt
  • MW
Unit size Price Item Code
50 mg $218.00 D630-10


Product Description:
DTSSP is a cross-linkingreagent reacting the amino groups with each other. Since DTSSP has at both ends of the molecule N-hydroxysuccinimide active ester to react selectively with the amino group, it can be conjugated a carrier protein to simple hapten or preparation labeled enzyme. BS3 takes a chemical structure having analkyl chain to the linker moiety. Meanwhile, DTSSP and DSP is capable of cleavage of the disulfide-introduced linker site that is easily reduced by the reducing agent. Further, since the active ester group having a sulfonic acid group is introduced to DTSSP and BS3, it may be carried out labeling reaction without using an organic solvent such as DMSO or DMF.

Technical info

Homo-bifunctional Reagents

Product Name Code Length (Å)
BS3 B574 8.9
DTSSP D630 8.5


Open References

1) J. V. Staros, "N-Hydroxysulfosuccinimide Active Ester: Bis(N-hydroxysulfosuccinimide) Ester of Two Dicarboxylic Acids Are Hydrophilic, Membrane-impermeant, Protein Cross-Linkers", Biochemistry198221, 3950.
2) S. M. Jung and M. Moroi, "Crosslinking of Platelet Glycoprotein Ib by N-Succinimidyl(4-azidophenyldithio)propionate and 3,3'-Dithiobis(sulfosuccinimidyl propionate)", Biochim. Biophys. Acta1983761, 152.
3) C. L. Swaim, J. B. Smith and D. L. Smith, "Unexpected Products From the Reaction of the Synthetic Cross-linker 3,3'-Dithiobis(sulfosuccinimidyl propionate), DTSSP with Peptides", J. Am. Soc. Mass Spectrom., 200415, 736.
4) P. Kao, S. Doerner, T. Schneider, D. Allara, P. Hauptmann and S. Tadigadapa, "A Micromachined Quartz Resonator Array for Biosensing Applications", J. Microekectromech. Syst., 200918(3), 522.
5) G. J. King, A. Jones, B. Kobe, T. Huber, D. Mouradov, D. A. Hume and I. L. Ross, "Identification of Disulfide-containing Chemical Cross-links in Proteins Using MALDI-TOF/TOF-Mass Spectrometry", Anal. Chem., 200880, 5036.

Handling and storage condition

Appearance: White to almost white powder
Purity (HPLC): ≧80.0 %
Solubility in water: To pass test (clear, colorless)
Handling and storage condition
0-5°C, Protect from moisture

Product Classification

Product Classification

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