Viability/Cytotoxicity Multiplex Assay Kit
Cell Proliferation / Cytotoxicity Assay KitSet of Cell Counting Kit-8 and Cytotoxicity LDH Assay Kit-WST (500 tests each)
Product codeCK17 Viability/Cytotoxicity Multiplex Assay Kit
|Unit size||Price||Item Code|
|500 tests||Cell Counting Kit-8
・WST-8, 1-Methoxy PMS
Cytotoxicity LDH Assay Kit-WST
55 ml x1
5.5 ml x1
27.5 ml x1
In order to confirm cytotoxicity, evaluation using only live cells or only dead cells as indicators may not be sufficiently reliable, and therefore, there are an increasing number of cases where experiments are supported by evaluating multiple indicators based on different measurement principles.
This kit is a set of two different indicators, Cell Counting Kit-8 (CCK-8) and Cytotoxicity LDH Assay Kit-WST (LDH Assay Kit). An assay manual is available to evaluate these two assays using the same sample.
When confirming cytotoxicity, evaluation using only live cells or only dead cells as indicators may not be sufficiently reliable, and therefore, more and more experiments are being supported by evaluation using multiple indicators based on different measurement principles.
Cell Counting Kit-8 and Cytotoxicity LDH Assay Kit-WST measure dehydrogenase activity and released amount of LDH as reflective of living cells and dead cells, respectively. According to the results with anticancer drug, the number of living cells was reduced, and the number of dead cells increased as the concentration of Mitomycin C increased. Interestingly, a low concentration of Mitomycin C (green frame) inhibits cell proliferation earlier than inducing cell death unlike a high concentration (red frame).
LDH Assay Kit: The activity of LDH leaked out of cells due to cell membrane damage can be confirmed as an indicator of dead cells.
Cell Counting Kit-8: Metabolic activity in living cells can be confirmed using NADH as an indicator.
This kit includes CCK-8 for measuring live cells and LDH Assay Kit for measuring dead cells, both of which can be evaluated by plate assay at the same absorbance wavelength. The same cell sample can be used to evaluate both live and dead cells at the same time, saving time and labor.
|Cell Type||Smaple||Title of reference||Reference|
|C3H10T1/2||Pirfenidone||XL413, a cell division cycle 7 kinase inhibitor enhanced the anti-fibrotic effect of pirfenidone on TGF-s1-stimulated C3H10T1/2 cells via Smad2/4）||S. Jin, et. al, Exp Cell Res., 2015, 339(2), 289-99|
|Human liver cell line L02||S17092
|Prolyl Oligopeptidase Inhibition Attenuates Steatosis in the L02 Human Liver Cell Line||Zhou D, et al., PLOS ONE, 2016, 11(10)|
(Ferric ammonium citrate)
|Iron Reduces M1 Macrophage Polarization in RAW264.7 Macrophages Associated with Inhibition of STAT1||Zhen-Shun Gan, et. al, Mediators of Inflammation, 2017|
|Atg5-dependent autophagy plays a protective role against methylmercury-induced cytotoxicity||Takanezawa Yasukazu, et. al, Toxicity Letters, 2016, 262, 135-141|
1) R. Watanabe, T. Kurose, Y. Morishige and K. Fujimori, "Protective Effects of Fisetin Against 6-OHDA-Induced Apoptosis by Activation of PI3K-Akt Signaling in Human Neuroblastoma SH-SY5Y Cells.", Neurochem. Res.., 2018, 43, (2), 488-499.
Handling and storage condition
|0-5°C,Protect from light|
Danger / harmful