Metabolism Promotion 2022
Why is cancer cell metabolism in the spotlight now?
Tumor development and progression require metabolic reprogramming of cancer cells. Cancer cells autonomously alter fluxes through various metabolic pathways to meet increased bioenergetic and biosynthetic demands as well as to reduce oxidative stress necessary for proliferation and survival*. Elucidating the triggers of these metabolic changes in cancer cells will lead to new possibilities for cancer therapy.
*Inmaculada Martinez-Reyes & Navdeep S. Chandel, Cancer metabolism: looking forward, Nature Reviews Cancer, 21, 669-680 (2021)
Promotional Products by Category
Category | Product | Unit | Price |
---|---|---|---|
Cellular |
ATP Assay Kit-Luminescence | 50 / 200 tests | $250.00 / $450.00 |
Glucose Assay Kit-WST (Colorimetric) | 50 / 200 tests | $185.00 / $400.00 | |
Lactate Assay Kit-WST (Colorimetric) | 50 / 200 tests | $297.00 / $714.00 | |
Glutamine Assay Kit-WST (Colorimetric) | 100 tests | $564.00 | |
Glutamate Assay Kit-WST (Colorimetric) | 100 tests | $513.00 | |
α-Ketoglutarate Assay Kit-Fluorometric | 100 tests | $660.00 | |
NAD/NADH Assay Kit-WST (Colorimetric) | 100 tests | $568.00 | |
NADP/NADPH Assay Kit-WST (Colorimetric) | 100 tests | $568.00 | |
Glycolysis/OXPHOS Assay Kit NEW | 50 tests | $480.00 | |
Nutrient |
Glucose Uptake Assay Kit-Green NEW | 50 / 100 tests | $150.00 / $380.00 |
Glucose Uptake Assay Kit-Blue / Red NEW | 100 tests | $400 | |
Amino Acid Uptake Assay Kit NEW | 20 / 100 tests | $160.00 / $450.00 | |
Cystine Uptake Assay Kit NEW | 20 / 100 tests | $180.00 / $500.00 |
Combination Assay with Metabolism Kits & Cell Counting Kit-8
Energy metabolism shifts by 2-Deoxy-D-glucose (2-DG) and oligomycin in HeLa cells
We inhibited glycolytic and mitochondrial oxidative phosphorylation in HeLa cells, respectively, and then evaluated metabolic shift and cell viability.
Step 1. | Confirm whether ATP production changes from glycolysis to mitochondrial oxidative phosphorylation |
Step 2. | Investigate changes in extracellular lactate levels as a measure of glycolytic activity |
Step 3. | Measure cell viability under the inhibition condition of each glycolysis and mitochondrial oxidative phosphorylation. |
In conclusion,
1. | Inhibition of the glycolytic pathway and oxidative phosphorylation in mitochondria, respectively, causes a metabolic pathway switch in HeLa cells. |
2. | Inhibition of both pathways, rather than inhibition alone, showed more effective anticancer activity. |
Step 1.
Step 2.
Related products
・ | ATP Assay Kit-Luminescence |
・ | Lactate Assay Kit-WST (Colorimetric) |
・ | Glycolysis/OXPHOS Assay Kit (ATP Assay Kit, Lactate Assay Kit, 2-DG, and oligomycin all-in-one kit) |
・ | Cell Counting Kit-8 Free in this promotion! |