03 Molecular Biology
05 Cell Staining

-Cellstain- PI solution

-Cellstain- PI solution

Dead Cell Staining

  • Product code
    P378  -Cellstain- PI solution
  • CAS No.
    25535-16-4(PI)
  • Chemical name
    3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide, solution
  • MW
    C27H34I2N4=668.39
Unit size Price Item Code
1 ml $65.00 P378-10
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Description

Product Description
Propidium iodide (PI) is an ethidium bromide analog that emits red fluorescence upon intercalation with double-stranded DNA. PI does not permeate viable cell membranes, but passes through disturbed cell membranes and stains the nuclei. PI is often used in combination with a fluorescein compound, such as Calcein-AM or FDA, for simultaneous staining of viable and dead cells. The excitation and emission wavelengths of PI-DNA complex are 535 nm and 615 nm, respectively.

 

Chemical Structure

Technical info

1.Prepare 10-50 μM PI solution with PBS or an appropriate buffer.a)
2.Add PI solution with 1/10 of the volume of cell culture medium to the cell culture.b)
3.Incubate the cell at 37oC for 10-20 min.
4.Wash cells twice with PBS or an appropriate buffer.
5.Observe the cells under a fluorescence microscope with 535 nm excitation and 615 nm emission filters.

a) Since PI may be carcinogenic, extreme care is necessary during handling. b) Or you may replac
e the culture medium with 1/10 concentration of PI buffer solution.

Staining Data

Fig. 2 Cell staining with PI

References

Open References

1) I. W. Taylor and B. K. Milthorpe, "An Evaluation of DNA Fluochromes, Staining Techniques, and Analysis for Flow Cytometry. I. Unperturebed Cellpopulations", J. Histochem. Cytochem., 1980, 28(11), 1224.
2) W. M. J. Vuist, F. V. Buitenen, M. A. De Rie, A. Hekman, P. Ruemke and C. J. M. Melief, "Potentiation by Interleukin 2 of Burkitt's Lymphoma Therapy with Anti-Pan B (Anti-CD19) Monoclonal Antibodies in a Mouse Xenotransplantation Model", Cancer Res., 1989, 49, 3783.
3) A. K. El-Naggar, J. G. Batsakis, K. Teague, L. Garnsey and B. Barlogie, "Single- and Double-stranded RNA Measurements by Flow Cytometry in Solid Neoplasms", Cytometry, 1991, 12, 330.
4) C. Souchier, M. Ffrench, M. Benchaib, R. Catallo and P. A. Bryon, "Methods for Cell Proloferation Analysis by Fluorescent Image Cytometry", Cytometry, 1995, 20, 203.
5)T. Yamazaki, H. Suzuki, S. Yamada, K. Ohshio, M. Sugamata, T. Yamada and Y. Morita, "Lactobacillus paracasei KW3110 Suppresses Inflammatory Stress-Induced Premature Cellular Senescence of Human Retinal Pigment Epithelium Cells and Reduces Ocular Disorders in Healthy Humans”, Int J Mol Sci, 2020, 21(14), 5091

Handling and storage condition

Specification
Appearance: Orange to red liquid
Dye content: To pass test
Handling and storage condition
-20°C, Protect from light
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Product Classification

Product Classification

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