15 Chromogen/Metal Indicator

Sodium bicinchoninate

Sodium bicinchoninate

Metal Indicator

  • Product code
    B037  Sodium bicinchoninate
  • CAS No.
  • Chemical name
    4,4'-Dicarboxy-2,2'-biquinoline, disodium salt
  • MW
Unit size Price Item Code
5 g $305.00 B037-10
5 g
Quantity may be adjusted within the cart.

Product Description

Sodium bicinchoninate is a Cu(I)- specific colorimetric reagent that is more sensitive than neocuproine. Its absorption maximum is at 562 nm (molar absorptivity: 7,900). In alkaline solution, Cu(II) is reduced by proteins to Cu(I), and then forms a purple complex with Sodium bicinchoninate. The protein detection range of this assay is 1-2,000 mg per ml.

Chemical Structure


Open References

1) K. Ueno and K. Kina, "Colloid Titration - A Rapid Method for the Determination of Charged Colloid", J. Chem. Education, 1985, 62, 627.
2) P. K. Smithm, R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olson and D. C. Klenk, "Measurement of Protein Using Bicinchoninic Acid", Anal. Biochem., 1985, 150, 76.
3) M. G. Redinbaugh and R. B. Turley, "Adaptation of the Bicinchoninic Acid Protein Assay for Use with Microtiter Plates and Sucrose Gradient Fractions", Anal. Biochem., 1986, 153, 267.

Handling and storage condition

Appearance: White to yellow powder
Solubility in water: To pass test (clear, colorless or pale yellow)
Molar absorptivity: ≧ 15,500 (around 332 nm)
Sulfated ash: 30.0 - 40.0 %
Blank (protein assay): To pass test
NMR spectrum: Authentic
Handling and storage condition
Ambient temperature

Product Classification

Product Classification

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