09 Cross-Linking Reagents

Sulfo-GMBS

Sulfo-GMBS

Cross-Linker

  • Product code
    S025  Sulfo-GMBS
  • CAS No.
    185332-92-7
  • Chemical name
    N-(4-Maleimidobutyryloxy)sulfosuccinimide, sodium salt
  • MW
    C12H11N2NaO9S=382.28
Unit size Price Item Code
50 mg S025-10

Description

Cross-linking Reaction

 

Product Description
Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature because it is necessary to maintain enzyme activity and antibody titers in the cross-linking reaction. Hetero-bifunctional cross-linking reagents with 3, 5, 7, or 10 linear carbon chains are available. These linear aliphatic chains act as spacers between the two reactive sites and their water-soluble reagents. They are more stable than the aromatic cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a wider pH range.

Conjugation of Macromolecules with Hetero-Bifunctional Cross-Linking Reagent

 

References

Open References

1) J. V. Staros, "N-Hydroxysulfosuccinimide Active Esters:Bis(N-hydroxysulfosuccinimide) Esters of Two Dicarboxylic acids Are Hydrophilic, Membrane-impermeant, Protein Cross-linkers", Biochemistry198221, 3950.
2) P. S. R. Anjaneyulu and James V. Staros, "Reactions of N-Hydroxysulfosuccinimide Active Esters", Int. J. Peptide Protein Res.198730, 117. 
3) Y. Fukami, K. Sato, K. Ikeda, K. Kamisango, K. Koizumi and T. Matsuno, "Evidence for Autoinhibitory Regulation of the c-src Gene Product a Possible Interaction Between the Src Homology 2 Domain and Autophosphorylation Site", J. Biol. Chem.1993268, 1132

Handling and storage condition

Specification
Appearance: White to slightly yellowish pink powder
Purity (HPLC): ≧ 90.0 %
Solubility in water: To pass test (clear, colorless or slightly yellow)
IR spectrum: Authentic
NMR spectrum: Authentic
Handling and storage condition
0-5°C
Contact
Price

Product Classification

Product Classification

Search word