08 Labeling Chemistry

ICG Labeling Kit - NH2

ICG Labeling Kit - NH<sub>2</sub>

Antibody / Protein Labeling

  • Suitable wavelength for In Vivo imaging
  • Quick and Easy Labeling to Antibodies : 1.5hr
  • High Recovery Rate : more than 90%
  • Product code
    LK31  ICG Labeling Kit - NH2
Unit size Price Item Code
3 samples LK31-10
3 samples ・NH2-Reactive ICG
・WS Buffer
・Reaction Buffer
・Filtration Tube
3 tubes
4 ml x1
500 μlx1
3 tubes


ICG Labeling Kit – NH2 is used primarily for the preparation of ICG (Iindocyanin green)-labeled antibody for near-infrared fluorescence imaging. ICG offers two remarkable properties:

1) ICG has a strong near-infrared fluorescence even after a few days under physiological conditions. The excitation and emission wavelength of the ICG-labeled proteins are 774 nm and 805 nm, respectively.
2) ICG has been used in clinical fields such as a hepatic deficiency testing. Therefore, ICG and ICG conjugates are materials suitable for in vivo imaging.

This kit contains all required compornents required for labeling, including storage buffer for conjugates. The labeling process is simple:. Add NH2-reactive ICG to protein solution on a filter membrane, and incubate at 37ºC, for 10 minutes. A filtration tube can remove excess ICG molecules.

Fig. 1 Fluorescein Labeling Process to IgG

♦ If the IgG solution contains other proteins with molecular weight greater than 10,000, such as serum albumin or gelatin, purify the IgG solution before labeling fluorescein with this kit. Commercially available antibody may contain BSA or gelatin as a stabilizer. 

Developer Dojindo Molecular Technologies, Inc.


Technical info

Fig. 2 Fluorescent Overlay Imaging

A mouse subcutaneous tumor model at 48 hours post injection of ICG labeled antibody(50 μg) shows clear evidence of selective accumulation in the tumor.



BALB/c nu nu (female, 11 weeks)

tumor cell:

HeLa (4 weeks after transplant in subcutaneous right axilla)


anti-integrin a2 antibody

measurement condition: 

Ex 785 nm, Em 845/55 nm/center/band), exposure time: 10 sec



Open References

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6) K. Inayama, S. Ito, N. Muguruma, Y. Kusaka, T. Bando, Y. Tadatsu, M. Tadatsu, K. Ii, S. Shibamura and K. Takesako, "Basic Study of an Agent for Reinforcement of Near-infrared Fluorescence on Tumor Tissue", Digestive and Liver Disease, 2003, 35, 88.
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8) S. Taoka, S. Ito, N. Muguruma, S. Hayashi, Y. Kusaka, K. Ii, K. Nakamura, K. Imaizumi, K. Takesako and S. Shibamura, "Reflected Illumination-type Imaging System for the Development of Infrared Fluorescence Endoscopy", Dig. Endosc.,1999, 11(4), 321.
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11) N. Muguruma, S. Ito, T. Bando, S. Taoka, Y. Kusaka, S. Hayashi, S. Ichikawa, Y. Matsunaga, Y. Tada, S. Okamura, K. Ii, K. Imaizumi, K. Nakamura, K. Takesako and S. Shibamura, "Labeled Carcinoembryonic Antigen Antibodies Excitable by Infrared Rays: a Novel Diagnostic Method for Micro Cancers in the Digestive Tract", Internal Medicine, 1999, 38(7), 537.
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Handling and storage condition

Handling and storage condition
0-5°C, Protect from moisture

Product Classification

Product Classification

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