09 Cross-Linking Reagents

FeBABE

Cross-Linking Reagents

Reagent for Biopolymer Function Analysis

Product code

F279　 FeBABE
CAS No.

186136-50-5
Chemical name

1-(p-Bromoacetamidobenzyl)ethylenediamine-N,N,N',N'-tetraacetic acid, iron(III)
MW

C₁₉H₂₁BrFeN₃O₉=571.13

Unit size	Price	Item Code
1 mg		F279-10

C of A

SDS download

Description

Product Description of BABEs
Bromoacetamidobenzyl-EDTA (BABE) is a chelate labeling reagent that conjugates with sulfhydryl groups. The iron chelate of BABE (FeBABE) is a unique tool for determining the three-dimensional structure of proteins and the binding structures of protein-protein or protein-DNA complexes. BABE adds EDTA moieties to proteins through their sulfhydryl groups. Once attached to a protein, FeBABE cuts a nearby peptide or DNA chain. The cleavage site is within 12 angstroms of the FeBABE binding site. Iron (II)-chelate cleaves a peptide or DNA chain in the presence of hydrogen peroxide. The cleavage reaction completes quickly: 10 seconds to 20 minutes of incubation is sufficient. The size of the cleaved fragment is analyzed with gel electrophoresis such as SDS-PAGE.

References

Open References

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8) R. Miyake, K. Murakami, J. T. Owens, D. P. Greiner, O. N. Ozoline, A. Ishihama and C. F. Meares, "Dimeric Association of Escherichia coli RNA Polymerase alfa subunits, studied by Cleavage of Single-Cysteine alfa Sununits Conjugated to Iron-(S)-1-(p-(Bromoacetamido)benzyl)ethylenediaminetetraacetate", Biochemistry, 1998, 37, 1344.
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