03 Molecular Biology

ES/iPS Differentiation Monitoring Kit - Human Endoderm

ES/iPS Differentiation Monitoring Kit - Human Endoderm

Endoderm Differentiation Monitoring

  • ELISA based endoderm differentiation monitoring
  • Continuous cell culture after measurement is possible
  • Great correlation with PCR, Flow cytometry and Immunostaining
  • Product code
    ES01  ES/iPS Differentiation Monitoring Kit - Human Endoderm
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Product Description :

One of the secretory proteins as in a culture medium was found to be a marker of the conversion level of ES and iPS to endodermal cells. (Published in: H. Iwashita, S. Kume, PloS ONE., 2013, 8(5): e64291)
The amount of this marker protein in the cell culture supernatant is determined by ELISA. It can be used to monitor the efficiency of differentiation of endodermal cells from ES/iPS cells. Since this kit is designed for the 96-well microplate format, it is suitable for multiple sample measurements, such as screening of inducers for differentiation or an optimization of culture conditions.

This product was developed by a collaborative work between Institute of Molecular Embryology and Genetics, Kumamoto University and Dojindo Laboratories.

RT-PCR Measurement of each Marker :

The secretory protein has a good correlation with Sox 17 and Foxa2 which are commonly used proteins as markers of endoderm differentiation.

Comparison with Conventional Techniques :

  Dojindo Kit PCR Immunostaining Flow Cytometry
Procedure 3 hours > 7 hours 1-2 days > 4 hours
Sample Supernatant mRNA Whole cells Whole cells
Cell Culture
Possible Impossible Impossible Impossible
Marker Marker Protein Sox17, Foxa2 Sox17, Foxa2



Measurement of Procedure :



Open References

H. Iwashita, N. Shiraki, D. Sakano, T. Ikegami, M. Shiga, K. Kume, S. Kume, PloS ONE., 2013, 8(5): e64291.

Handling and storage condition

Handling and storage condition

Product Classification

Product Classification

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