ES/iPS Differentiation Monitoring Kit - Human Endoderm
Endoderm Differentiation Monitoring
- ELISA based endoderm differentiation monitoring
- Continuous cell culture after measurement is possible
- Great correlation with PCR, Flow cytometry and Immunostaining
Product codeES01 ES/iPS Differentiation Monitoring Kit - Human Endoderm
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Product Description :
One of the secretory proteins as in a culture medium was found to be a marker of the conversion level of ES and iPS to endodermal cells. (Published in: H. Iwashita, S. Kume, PloS ONE., 2013, 8(5): e64291)
The amount of this marker protein in the cell culture supernatant is determined by ELISA. It can be used to monitor the efficiency of differentiation of endodermal cells from ES/iPS cells. Since this kit is designed for the 96-well microplate format, it is suitable for multiple sample measurements, such as screening of inducers for differentiation or an optimization of culture conditions.
This product was developed by a collaborative work between Institute of Molecular Embryology and Genetics, Kumamoto University and Dojindo Laboratories.
RT-PCR Measurement of each Marker :
The secretory protein has a good correlation with Sox 17 and Foxa2 which are commonly used proteins as markers of endoderm differentiation.
Comparison with Conventional Techniques :
|Dojindo Kit||PCR||Immunostaining||Flow Cytometry|
|Procedure||3 hours||> 7 hours||1-2 days||> 4 hours|
|Sample||Supernatant||mRNA||Whole cells||Whole cells|
|Marker||Marker Protein||Sox17, Foxa2||Sox17, Foxa2||
Measurement of Procedure :
H. Iwashita, N. Shiraki, D. Sakano, T. Ikegami, M. Shiga, K. Kume, S. Kume, PloS ONE., 2013, 8(5): e64291.
Handling and storage condition