ROS Research
1. ROS Assay Kit - Photo-oxidation Resistant DCFH-DA
Resistant to Photo-oxidation
Has photooxidation ever interfered with your experiments?
Unstimulated HeLa cells were stained with regular DCFH-DA or Photo-oxidation Resistant DCFH-DA and observed with excitation light every 5 minutes for fluorescence imaging.
Compatible wh Immunostaining
Co-staining with anti-protein immunostain
After adding hydrogen peroxide, HeLa cells were stained with Photo-oxidation resistant DCFH-DA and mitochondrial marker Tom 20. As a result, the intracellular ROS level and mitochondrial morphology were clearly observed at the same time, which is very difficult for the existing ROS probe.
2. ROS Assay Kit - Highly Sensitive DCFH-DA
Super High-Sensitive ROS Detection
Comparison of Sensitivity: Microscope
Hydrogen peroxide (H2O2)-treated HeLa cells (1×104 cells/ml) were stained with DCFH-DA or the ROS Assay Kit-Highly Sensitive DCFH-DA, and the detectability of intracellular ROS was compared between two detection kits. Results indicated that the ROS Assay Kit-Highly Sensitive DCFH-DA was better at high-sensitivity detection of intracellular ROS than regular DCFH-DA in high-sensitivity detection of intracellular ROS was better than DCFH-DA.
Ex: 450-490 nm, Em: 500-550 nm (GFP filter), Scale bar: 50 μm
3. Compare Dojindo's ROS Assay Kits with Others
|
Product |
Unit |
Code |
|
100 tests |
R253-10 |
|
|
100 tests |
R252-10 |
4. Application Data
Simultaneously evaluation of mitochondrial superoxide and intracellular total ROS
HeLa cells were washed with HBSS and co-stained with mtSOX Deep Red - Mitochondrial Superoxide Detection and ROS Assay Kit -Highly Sensitive DCFH-DA- (Total ROS detection), and separately treated with mitochondrial superoxide inducer Antimycin or hydrogen peroxide. As a result, intracellular total ROS and mitochondrial superoxide were observed separately.
General Protocol
Treated with H2O2
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Treated with Antimycin
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＜Imaging Conditions＞(Confocal microscopy)
Intracellular ROS: Ex = 488, Em = 490-520 nm
mtSOX: Ex = 633 nm, Em = 640-700 nm
Scale bar: 10 μm
