Understanding GPX4 Function in Cancer Cell Survival [Feb. 25, 2025]
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| GPX4, a selenoprotein, inhibits lipid oxidation and protects cancer cells from oxidative damage and ferroptosis. Targeting GPX4 has emerged as a promising strategy, offering new avenues for cancer treatment. This Science Note introduces recent research papers on cancer and GPX4. | ||
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Breast cancer secretes anti-ferroptotic MUFAs and depends on selenoprotein synthesis for metastasis Highlighted technique: Measurement of lipid peroxidation is a key indicator of ferroptosis and has been widely reported in various research publications. C11 BODIPY is commonly used to detect lipid peroxidation, while Liperfluo, with its higher specificity for lipid peroxides, serves as a valuable tool for ferroptosis research. |
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Dysregulated cholesterol homeostasis results in resistance to ferroptosis increasing tumorigenicity and metastasis in cancer Highlighted technique: In vivo experiments are essential for assessing cancer metastasis, but they are time-consuming. For rapid evaluation, Migration Assays such as those described in this paper can be useful. |
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PRDX6 dictates ferroptosis sensitivity by directing cellular selenium utilization Highlighted technique: This paper describes an affinity purification method for GPX4 analysis. A more detailed purification and evaluation method can be found in the authors' report in Cell Reports Methods. |
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