Dojindo offers various assay kits and reagents to evaluate the key indicators of Cell Function Analysis.
Information on more than 100 products are available in Analytical Reagents for Cellular Function
We hope it would be helpful to advance your research on Cell Function Analysis.
Cellular function analysis
What is Cellular function analysis?
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Cellular function analysis has become an important research area in medical field, including elucidation of disease mechanisms and development of the pharmaceuticals. It has also become a focus for cosmetics industry.
Especially, the intracellular metabolism, cellular senescence, and the mitochondrial function are known to be closely related to each other. Thus, it is recommended to use the multiple indicators for the analysis.
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Intracellular Metabolism
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Understanding the intracellular metabolism, such as glycolysis, the TCA cycle and mitochondrial electron transport, is essential for analyzing the status of the cell.
We offer the ready-to-use kits and reagents to analyze the intracellular metabolism with various indicators evaluating the metabolites and nutrients uptake, including glucose, lactate, NAD(P)/NAD(P)H, and glutamine/cystine, as well as the mitochondrial function, including mitochondrial membrane potential, and OCR (Oxygen Consumption Rate).
Related product
Glycolysis/OXPHOS Assay | Glycolysis/OXPHOS Assay Kit | G270 |
Glycolysis/Mitochondrial Membrane Potential Assay | Glycolysis/JC-1 MitoMP Assay Kit | G272 |
ATP Measurement | ATP Assay Kit-Luminescence | A550 |
ADP/ATP Ratio Assay | ADP/ATP Ratio Assay Kit-Luminescence | A552 |
Glucose Metabolism Assay | Glucose Assay Kit-WST | G264 |
Glutamine Assay | Glutamine Assay Kit-WST | G268 |
Glutamate Assay | Glutamate Assay Kit-WST | G269 |
α-Ketoglutaric Acid Measurement | α-Ketoglutarate Assay Kit-Fluorometric | K261 |
Lactic Acid Measurement | Lactate Assay Kit-WST | L256 |
NAD/NADH Assay | NAD/NADH Assay Kit-WST | N509 |
NADP/NADPH Assay | NADP/NADPH Assay Kit-WST | N510 |
Glucose uptake capacity assay | Glucose Uptake Assay Kit-Blue | UP01 |
Glucose uptake capacity assay | Glucose Uptake Assay Kit-Green | UP02 |
Glucose uptake capacity assay | Glucose Uptake Assay Kit-Red | UP03 |
Amino Acid Uptake Assay | Amino Acid Uptake Assay Kit | UP04 |
Cystine Uptake Assay | Cystine Uptake Assay Kit | UP05 |
Fatty Acid Uptake Assay | Fatty Acid Uptake Assay Kit | UP07 |
Mitochondria
Dojindo offers a broad range of reagents and kits for the research on mitochondria, including Mitophagy Detection, Oxygen Consumption Rate Assay, ROS Assay/Detection Kit, as well as the Assay/Detection of lipid peroxidation caused by the ROS.
Mitochondrial membrane potential (MtMP) dependent fluorescent probes for mitochondria staining are also available.
Related product
OCR Plate Assay Kit | Extracellular OCR Plate Assay Kit | E297 |
Glucose Metabolism Assay | Glucose Assay Kit-WST | G264 |
Lactic Acid Measurement | Lactate Assay Kit-WST | L256 |
Mitochondrial Membrane Potential Detection | JC-1 MitoMP Detection Kit | MT09 |
Mitochondria Research | MT-1 MitoMP Detection Kit | MT13 |
Mitophagy Detection | Mitophagy Detection Kit | MD01 |
Mitophagy Detection | Mtphagy Dye | MT02 |
Mitochondrial Staining | MitoBright LT Green | MT10 |
Mitochondrial Staining | MitoBright LT Red | MT11 |
Mitochondrial Staining | MitoBright LT Deep Red | MT12 |
Mitochondrial staining | MitoBright IM Red for Immunostaining | MT15 |
Mitochondrial Superoxide Detection | MitoBright ROS Deep Red - Mitochondrial Superoxide Detection | MT16 |
Mitochondrial Iron Detection | Mito-FerroGreen | M489 |
Mitochondrial Singlet Oxygen Imaging | Si-DMA for Mitochondrial Singlet Oxygen Imaging | MT05 |
Mitochondrial Lipid Peroxide Detection | MitoPeDPP | M466 |
Cellular Senescence
Understanding the apoptosis, necrosis, autophagy and cellular senescence is critical for Cell Function Analysis. Especially, the cellular senescence has been attracting the attention in various fields since the report of the SASP (senescence-associated secretory phenotype), and the stem cell senescence.
We offer four types of kits and reagents with different indicators and methods.
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■ Increased expression of SA-β-Gal
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■ Accumulation of DNA damage
■ Nucleolar hypertrophy
Related product
Senescence Cell Detection | Cellular Senescence Detection Kit - SPiDER-βGal | SG03 |
Senescence Cell Detection (for Microplate) | Cellular Senescence Plate Assay Kit - SPiDER-βGal | SG05 |
Experiments using multiple indicators
■ Example: Observing the intracellular metabolic changes associated with cellular senescence
In cellular senescence induced cells, an increased expression of SA-β-gal and the irreversible cell-cycle arrest are observed, and those cells with accumulation of DNA damage shift the energy metabolism to glycolysis as the consequence of the decline in mitochondrial function.
The changes in cell function were evaluated with doxorubicin (DOX) induced senescent A549 cells by conducting the experiments using multiple indicators.
Related products
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Cell Proliferation/ Cytotoxicity Assay
Cell Proliferation / Cytotoxicity Assay, CCK-8, WST-8 Cell Counting Kit-8 CK04 Cytotoxicity Assay Cytotoxicity LDH Assay Kit-WST CK12 Cell Proliferation / Cytotoxicity Assay Kit Viability/Cytotoxicity Multiplex Assay Kit CK17 Cell Count Normalization Cell Count Normalization Kit C544 Autophagy
Autophagy (Autolysosome) Detection DALGreen - Autophagy Detection D675 Autophagy (Autophagosome) Detection DAPGreen - Autophagy Detection D676 Autophagy (Autophagosome) Detection DAPRed - Autophagy Detection D677 Oxidative Stress
ROS Detection ROS Assay Kit -Highly Sensitive DCFH-DA- R252 Photo-oxidation Resistant ROS Assay Kit ROS Assay Kit -Photo-oxidation Resistant DCFH-DA- R253 Mitochondrial Superoxide Detection MitoBright ROS Deep Red - Mitochondrial Superoxide Detection MT16 Mitochondrial Singlet Oxygen Imaging Si-DMA for Mitochondrial Singlet Oxygen Imaging MT05 Lipid Peroxide Detection Liperfluo L248 Lipid Peroxidation Assay Lipid Peroxidation Probe -BDP 581/591 C11- L267 Mitochondrial Lipid Peroxide Detection MitoPeDPP M466 Intracellular Iron Ion Measurement FerroOrange F374 Mitochondrial Iron Detection Mito-FerroGreen M489 Glutathione Quantification GSSG/GSH Quantification Kit G257 Lysosome
Lysosomal Acidic pH Detection Kit Lysosomal Acidic pH Detection Kit L266 Lysosome staining reagent (Deep Red) LysoPrime Deep Red - High Specificity and pH Resistance L264 Lysosomal Acidic pH Detection pHLys Red - Lysosomal Acidic pH Detection L265
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Endocytosis
Endocytosis Detection ECGreen-Endocytosis Detection E296 Other Organelles
Cell Membrane Staining PlasMem Bright Green P504 Cell Membrane Staining PlasMem Bright Red P505 Nucleolus Fluorescent Staining Nucleolus Bright Green N511 Nucleolus Fluorescent Staining Nucleolus Bright Red N512 Exosome
Exosome Membrane Fluorescent Staining ExoSparkler Exosome Membrane Labeling Kit-Green EX01 Exosome Membrane Fluorescent Staining ExoSparkler Exosome Membrane Labeling Kit-Red EX02 Exosome Membrane Fluorescent Staining ExoSparkler Exosome Membrane Labeling Kit-Deep Red EX03 Exosome Protein Fluorescent Staining ExoSparkler Exosome Protein Labeling Kit-Green EX04 Exosome Protein Fluorescent Staining ExoSparkler Exosome Protein Labeling Kit-Red EX05 Exosome Protein Fluorescent Staining ExoSparkler Exosome Protein Labeling Kit-Deep Red EX06 Exosome Isolation ExoIsolator Exosome Isolation Kit EX10 Filter for exosome isolation kit ExoIsolator Isolation Filter EX11 Lipid Droplet
Lipid Droplet Staining Lipi-Blue LD01 Lipid Droplet Staining Lipi-Green LD02 Lipid Droplet Staining Lipi-Red LD03 Lipid Droplet Staining Lipi-Deep Red LD04 Lipid Droplet Assay Lipid Droplet Assay Kit - Blue LD05 Lipid Droplet Assay Lipid Droplet Assay Kit - Deep Red LD06
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Dojindo offers various assay kits and reagents to evaluate the key indicators of Cell Function Analysis.
Information on more than 100 products are available in Analytical Reagents for Cellular Function.
We hope it would be helpful to advance your research on Cell Function Analysis.