Reverse Electron Transfer in Age-Related Diseases

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This article focusing on reverse electron transfer (RET) at mitochondrial complex I, which trigers age-related disease. These findings may help to understand the relationship between senescence and metabolism through mitochondrial dysfunction.
Reverse electron transfer is activated during aging and contributes to aging and age-related

Suman Rimal, et. al., EMBO Reports, e55548 (2023)

Point of Interest
     - RET is activated in aged Drosophila and Drosophila models of Alzheimer's disease (AD).
     - Inhibition of RET using the small molecule drug CPT or by knocking down the mitochondrial complex I subunit NDUFS3 extends the lifespan in both Drosophila and mice.
     - RET inhibition rescues AD-related disease phenotypes in Drosophila and mouse models.
     - RET activity is also observed in human induced pluripotent stem cell (iPSC) models of AD.            
Related Techniques
           Cellular senescence detection (Live cell imaging or FCM) Cellular Senescence Detection Kit HOT
           Cellular senescence detection (Plate reader) Cellular Senescence Plate Assay Kit HOT
           Oxygen consumption rate assay Extracellular OCR Plate Assay Kit
           NAD/NADH assay NAD/NADH Assay Kit
           Mitochondrial membrane potential detection JC-1 MitoMP Detection Kit / MT-1 MitoMP Detection Kit
           Mitochondrial superoxide detection MitoBright ROS Deep Red - Mitochondrial Superoxide Detection
           ​​Total ROS detection Higher sensitivity or Compatible with Co-staining for Immunostaining HOT
Related Applications

Metabolic shift to glycolysis in senescenct cells

 

NAD(+) levels decline during the aging process, causing defects in nuclear and mitochondrial functions and resulting in many age-associated pathologies*. Here, we try to redemonstrate this phenomenon in the doxorubicin (DOX)-induced cellular senescence model with a comprehensive analysis of our products.                       

*S. Imai, et al., Trends Cell Biol, 2014, 24, 464-471

Products in Use
① DNA Damage Detection Kit - γH2AX
② Cellular Senescence Detection Kit - SPiDER-βGal
③ NAD/NADH Assay Kit-WST
④ JC-1 MitoMP Detection Kit
⑤ Glycolysis/OXPHOS Assay Kit、Lactate Assay Kit-WST

 

 

 

 

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