Cell Counting Kit-8
~December
2018 Publications~

Upregulated circular RNA circ_0030235 predicts unfavorable prognosis in pancreatic ductal adenocarcinoma and facilitates cell progression by sponging miR-1253 …

Y Xu, Y Yao, P Gao, Y Cui - Biochemical and Biophysical Research …, 2018

… Cell transfection was performed using Lipofectamine 3000 (Invitrogen, USA).
The cells were subjected to further analysis at 48 h post-transfection. 2.5.
Cell proliferation experiments. The capacity of cell proliferation was analyzed …

MYC upregulated LINC00319 promotes human acute myeloid leukemia (AML) cells growth through stabilizing SIRT6

Y Zhang, Z Huang, F Sheng, Z Yin - Biochemical and Biophysical Research …, 2018

… Afterwards, cells were added with 10 µl Cell Counting Kit-8 (CCK-8; Dojindo,
Kumamoto, Japan) solution and furtherly incubated at 37 °C for 2 h. After
that, absorbance of each well was measured at 450 nm by using Multiskan …

Photosensitizer Loaded Multifunctional Chitosan Nanoparticles for Simultaneously in Situ Imaging, High Efficient Bacterial Biofilms Eradication and Tumor Ablation

L Sun, W Jiang, H Zhang, Y Guo, W chen, Y Jin… - ACS Applied Materials & …, 2018

Page 1. Photosensitizer Loaded Multifunctional Chitosan Nanoparticles for
Simultaneously in Situ Imaging, High Efficient Bacterial Biofilms Eradication and Tumor
Ablation Lin Suna,#, Wenya Jianga,#, Hengrui Zhanga, b, Yishun …

Highly tumor-specific DNA nanostructures discovered by in vivo screening of a nucleic acid cage library and their applications in tumor-targeted drug delivery

KR Kim, SJ Kang, AY Lee, D Hwang, M Park, H Park… - Biomaterials, 2018

… were additionally incubated for 24 h. Finally, cell viability was analyzed by
using a cell counting assay kit (CCK-8, Dojindo, Molecular Technologies …
treated with DOX@L-Tds and DOX were incubated for 24 h. The cell viability …

LncRNA SNHG14 promotes the progression of cervical cancer by regulating miR-206/YWHAZ

N Ji, Y Wang, G Bao, J Yan, S Ji - Pathology-Research and Practice, 2018

… 2.4. CCK-8 assay. The growth of CC cells was assessed by the use of Cell Counting
Kit-8 (CCK-8; Dojindo Molecular Technologies). The transfected cells (1 × 10 3
cells/well) were seeded in 96-well plates and then incubated …

Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome

Y Li, Q Zheng, D Sun, X Cui, S Chen, A Bulbul, S Liu… - Journal of Cellular …, 2018

Cell Counting Kit 8 (CCK8; Dojindo Laboratories, Kumamoto, Japan) was used to assess
the proliferation rate of KGN cells. Ten microliter of CCK8 solution was added to 90 µl
DMEM/F12 medium and incubated for 2 hr at 37°C. Then, the OD450 nm was recorded …

An efficient prodrug-based nanoscale delivery platform constructed by water soluble eight-arm-polyethylene glycol-diosgenin conjugate

C Li, J Dai, D Zheng, J Zhao, Y Tao, J Lei, X Xi, J Liu - Materials Science and …, 2018

… Gibco Dulbecco's Phosphate-Buffered Saline (DPBS), Gibco Dulbecco's Modified Eagle's
Medium (DMEM), penicillin and streptomycin were all bought form Invitrogen Hyclone.
The Cell-Counting Kit-8 (CCK8) kit was obtained by the Dojindo Laboratories …

A nano-complex system to overcome antagonistic photo-chemo combination cancer therapy

C Lim, J Moon, T Sim, WR Won, ES Lee, YS Youn… - Journal of Controlled …, 2018

… were purchased from Welgene (Seoul, Korea). Cell Counting Kit-8 (CCK-8) was
obtained from Dojindo Molecular Technologies (Tokyo, Japan). 2.2. Measurement
of size and zeta potential of nanoparticles at different pH values …

ECT2/PSMD14/PTTG1 axis promotes the proliferation of glioma through stabilizing E2F1

T Zhi, K Jiang, X Xu, T Yu, F Zhou, Y Wang, N Liu… - Neuro-Oncology, 2018

… Finally, cells were visualized under a fluorescent microscope. Cell viability assay Cell
viability was measured in duplicate samples with a Cell Counting Kit-8 (CCK-8, Dojindo,
Japan) in three independent experiments. Stably transfected glioma cells …

Delayed Increase in Near-Infrared Fluorescence in Cultured Murine Cancer Cells Labelled with Oxygen-Doped Single-Walled Carbon Nanotubes

S Sekiyama, M Umezawa, Y Iizumi, T Ube, T Okazaki… - Langmuir, 2018

… Cytotoxicity Assay. To evaluate cell viability, Colon-26 cells were cultured
in 96-well plate with the o-SWCNT-PEG (0-3.2 µg/mL) for 24 h, and then
treated with WST-1 solution (Cell Counting Kit-8; Dojindo Laboratories …

[HTML] Propofol alleviates hypoxia-induced nerve injury in PC-12 cells by up-regulation of microRNA-153

M He, H Sun, J Pang, X Guo, Y Huo, X Wu, Y Liu, J Ma - BMC Anesthesiology, 2018

… at 37 °C overnight. Then, after treatments, 10 µL of Cell Counting Kit-8 (CCK-8)
solution, provided by the Dojindo Molecular Technologies (Gaithersburg, MD, USA)
was added into the culture medium. Cells were incubated at …

miR-222/GAS5 is involved in DNA damage and cytotoxic effects induced by temozolomide in T98G cell line

H Huang, R Jiang, Z Lian, W Zhang, Z Hu, D Hu - Journal of Applied Toxicology, 2018

kit-8 analysis The CCK-8 assay was used to measure cell proliferation. A
total of 2 × 105 cells/per well were seeded on to 96-well culture plates and
incubated at 37°C for up to 6 days. Subsequently, 100 µL fresh media …

Chondroitin sulfate N-acetylgalactosaminyltransferase-2 deletion alleviates lipoprotein retention in early atherosclerosis and attenuates aortic smooth muscle cell …

IM Adhikara, K Yagi, DS Mayasari, K Ikeda, H Kitagawa… - … and Biophysical Research …, 2018

… Cell proliferation was assessed by the Cell Counting Kit-8 assay according to the
manufacturer's recommendations (Dojindo, Kumamoto, Japan). Data are presented as
means of three independent experiments (each using six replicate wells) ± SEM …

[PDF] Low expression of microRNA-1266 promotes colorectal cancer progression via targeting FTO

XP Shen, X Ling, H Lu, CX Zhou, JK Zhang, Q Yu - European Review for Medical and …, 2018

… 10 µL of CCK-8 so- lution (cell counting kit-8, Dojindo, Kumamoto, Japan)
were added in each well after cell culture for 6, 24, 48, 72 and 96 h,
respectively. The ab- sorbance at 450 nm of each sample was measured …

[PDF] Low expression of lncRNA MEG3 promotes the progression of oral squamous cell carcinoma by targeting miR-21

LL Zhang, D Hu, LH Zou - European Review for Medical and Pharmacological …, 2018

Cell Counting Kit-8 (CCK-8) Assay Cells in logarithmic growth phase were
select- ed seeded into 96-well plates at a density of 1×106/ mL. After 6 h,
10 µL CCK-8 solution (Dojindo, Kumamoto, Japan) was added to each well …

Proanthocyanidins inhibit osteoclast formation and function by inhibiting the NF-?B and JNK signaling pathways during osteoporosis treatment

W Zhu, Z Yin, Q Zhang, S Guo, Y Shen, T Liu, B Liu… - … and Biophysical Research …, 2018

… Sigma-Aldrich, USA). The Cell Counting Kit-8 (CCK-8) was obtained from Dojindo
Molecular Technology (Japan). Soluble mouse recombinant M-CSF and RANKL were
purchased from R&D Systems (USA). The Acid Phosphatase …

[PDF] LncRNA SNHG8 promotes the development and chemo-resistance of pancreatic adenocarcinoma

Y Song, L Zou, J Li, ZP Shen, YL Cai, XD Wu - European Review for Medical and …, 2018

Cell Counting Kit-8 (CCK-8) Assay The transfected cells were first seeded
into 96-well plates at a density of 1×104/µL. After cul- turing for 0, 24, 36,
48 and 72 h, 10 µL CCK-8 solution (Dojindo, Kumamoto, Japan) was added …

[PDF] MiR-210 knockdown promotes the development of pancreatic cancer via upregulating E2F3 expression

FB SUN, Y Lin, SJ LI, J Gao, B Han, CS ZHANG - European Review for Medical and …, 2018

Cell Counting Kit-8 (CCK-8) Assay Transfected cells were seeded into 96-well pla- tes
at a density of 1×105/ml. A total of 10 µl CCK-8 solution (Dojindo, Kumamoto, Japan)
was added in each well after culture for 24, 48 and 72 h, re- spectively …

Overexpression of CLN3 contributes to tumour progression and predicts poor prognosis in hepatocellular carcinoma

Y Xu, H Wang, Y Zeng, Z Shen, Z Xie, F Chen… - Surgical Oncology, 2018

… S2). 2.7.1. Cell proliferation assay. The cell proliferation assay was performed using
Cell Counting Kit-8 solution (Dojindo Laboratories) according to the manufacturer's
instructions. Briefly, the cells were seeded at a density of …

Knockdown of long non-coding RNA TUG1 suppresses nasopharyngeal carcinoma progression by inhibiting epithelial-mesenchymal transition (EMT) via the …

W Qian, Z Ren, X Lu - Biochemical and Biophysical Research …, 2018

… 2.5. CCK8 analysis. Cell Counting Kit-8 (CCK8) (Dojindo, Japan) was used to
measure the proliferative capacity of NPC cells. Briefly, the cells were seeded on
96-well plates after transfection. CCK8 was added to the plates …

[PDF] LncRNA GIHCG promotes development of ovarian cancer by regulating microRNA-429

N Yao, L Yu, B Zhu, HY Gan, BQ Guo - European Review for Medical and …, 2018

Cell Counting Kit-8 (CCK-8) Assay OC cells were seeded into 96-well plates with 2×103
cells per well. 10 µL of CCK-8 solution (cell counting kit-8, Dojindo, Kumamoto, Japan)
were added in each well. 4 hours later, fresh medium was replaced for 1-h incubation …

Cigarette smoking aggravates bleomycin-induced experimental pulmonary fibrosis

LL Zhou, M Wang, F Liu, YZ Lu, LJ Song, L Xiong… - Toxicology Letters, 2018

… The Cell Counting Kit-8 was purchased from Dojindo (Kumamoto, Japan) …
2.11. Cell proliferation testing. The proliferation assay for PMCs and
fibroblasts were performed using the Cell Counting Kit-8 (Dojindo, Kumamoto …

[HTML] Fucoidan-Manganese Dioxide Nanoparticles Potentiate Radiation Therapy by Co-Targeting Tumor Hypoxia and Angiogenesis

SW Shin, W Jung, C Choi, SY Kim, A Son, H Kim… - Marine Drugs, 2018

… To determine the optimal nanoparticle dosage, the cytotoxicity of Fuco-MnO
2 -NPs was assessed in human pancreatic cancer cell lines (AsPC-1 and
BxPC-3) using a cell counting kit 8 (CCK-8; Dojindo, Mashiki-machi, Japan) …

UV-B-activated B16 melanoma cells or HaCaT keratinocytes increase melanogenesis via ANGPTL 2 induction, an activity antagonized by Chrysanthemum extract

G Satou, D Maji, T Isamoto, Y Oike, M Endo - Experimental Dermatology, 2018

… instructions. 2.7 | Cell viability analysis Cells were counted using a Cell Counting
Kit-8 (CCK8; Dojindo Laboratories, Kumamoto, Japan), according to the manufacturer's
instructions. Briefly, B16 cells were plated in 96-well plates …

[PDF] Apatinib inhibits tumor growth and angiogenesis in PNET models

S Wu, JJ Zhou, J Guo, Z Hua, JC Li, Z Wang - Endocrine Connections, 2018

… Page 10. Cell viability assays were determined by using Cell Counting
Kit-8 (CCK-8) (WST-8, Dojindo, Kumamoto, Japan), according to the
supplier recommendations. Cells were cultured in 96-well plates (3x103 …

[PDF] The long non-coding RNA-ROR promotes osteosarcoma progression by targeting miR-206

D Fei, G Sui, Y Lu, L Tan, Z Dongxu, K Zhang - Journal of Cellular and Molecular …, 2018

… 2.5 | Cell proliferation assay Cell viability was detected using Cell Counting Kit-8
(CCK-8; Dojindo Molecular Technologies, Inc., Kumamoto, Japan). Briefly, transfected
cells were seeded into 96-well plates at a density of 5,000 per well …

Circular RNA HIPK3 promotes glioma progression by binding to miR-124-3p

D Hu, Y Zhang - Gene, 2018

… well plates. After cell cultured for 24 h, 48 h, 72 h and 96 h, 10 µl of Cell-Counting
Kit 8 (CCK8; Dojindo Laboratories, Kumamoto, Japan) reagent was added in each
well according to the manufacturer's instructions. Two hours …

[PDF] CCN1/Cyr61 Stimulates Melanogenesis through Integrin a6b1, p38 MAPK, and ERK1/2 Signaling Pathways in Human Epidermal Melanocytes

Z Xu, L Chen, M Jiang, Q Wang, C Zhang, LF Xiang - 2018

… The inset shows representative Western blots. (c) Cell viability of NHEMs after treatment
with different dosages of CCN1 was evaluated with a CCK8 (Cell Counting Kit 8) assay
(Dojindo, Kamimashiki-gun, Japan). Cell viability was presented as percentage of control …

Inhibitory Effect of Damulin B from Gynostemma pentaphyllum on Human Lung Cancer Cells

SF Xing, LH Liu, ML Zu, M Lin, XF Zhai, XL Piao - Planta Medica, 2018

… compounds in our laboratory [21], [23], [24], [25]. According to the Cell Counting
Kit-8 (CCK-8) assay, they manifested strong activities against A549 cells in a
dose-dependent manner ([Fig. 1 c]). Their 50% inhibitory concentration (IC …

Downregulation of TIMP2 attenuates sepsis-induced AKI through the NF-?b pathway

YM Li, J Zhang, LJ Su, JA Kellum, ZY Peng - … Acta (BBA)-Molecular Basis of Disease, 2018

… 2.10. Cell viability. TIMP2-S and TIMP2-C HK-2 cells were seeded in 96-well plates at
a density of 5× 10 3 cells/well with LPS for 4 to 60 h. Cell viability was detected by the
Cell Counting Kit-8 (Dojindo Molecular Technologies, Kumamoto, Japan) …

[HTML] Involvement of C-terminal truncation mutation of kinesin-5 in resistance to kinesin-5 inhibitor

E Saeki, S Yasuhira, M Shibazaki, H Tada, M Doita… - PLOS ONE, 2018

… After the treatment, the cells were incubated with fresh medium containing
10% of Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) for an additional
few hours, and then the absorbance at 450 nm was measured using a …

microRNA-148a-3p inhibited the proliferation and epithelial–mesenchymal transition progression of non-small-cell lung cancer via modulating Ras/MAPK/Erk …

Q Xie, Z Yu, Y Lu, J Fan, Y Ni, L Ma - Journal of cellular physiology, 2018

… After an overnight incubation, the cells were transfected with the RNAs
(miR-148a-3p or control) for 48 hr. Subsequently, the medium was removed
and cell counting solution (Cell Counting Kit-8 [CCK-8]; Dojindo

[PDF] PSPH Mediates the Metastasis and Proliferation of Non-small Cell Lung Cancer through MAPK Signaling Pathways

L Liao, M Ge, Q Zhan, R Huang, X Ji, X Liang, X Zhou - International Journal of …, 2019

… Cell proliferation capacity was performed by cell counting kit-8 (CCK-8),
colony formation assays and cell cycle analysis … using WST-8 (2-(2-methoxy-
4-nitrophenyl)-3-(4-nitro- phenyl) -5-(2,4-disulfophenyl) -2H-tetrazolium) …

Donor T-cell-derived interleukin-22 promotes thymus regeneration and alleviates chronic graft-versus-host disease in murine allogeneic hematopoietic cell transplant

B Pan, F Zhang, Z Lu, L Li, L Shang, F Xia, R Fu, M Xu… - International …, 2019

… 2.13. Cell viability. Viability of cells was measured with a Cell Counting Kit-8 (CCK-8,
CK04) from DOJINDO (Tokyo, Japan). 2.14. Immunohistochemistry. To detect expression
of Aire, immunohistochemistry was performed on paraffin slides of thymus …

MicroRNA-506 inhibits the proliferation and invasion of mantle cell lymphoma cells by targeting B7H3

X Zhu, J Wang, M Zhu, Y Wang, S Yang, X Ke - Biochemical and Biophysical …, 2018

… Assays were performed three times. 2.6. Cell proliferation assays. Cell proliferation
was determined using a Cell Counting Kit-8 assay (CCK-8; Dojindo, Japan) in
accordance with the manufacturer's instructions. Cells in the logarithmic …

Fluvastatin-mediated down-regulation of SATB1 affects aggressive phenotypes of human non-small-cell lung cancer cell line H292

HY Xu, JX Xue, H Gao, FF Na, H Li, T Zhang, Y Lu - Life Sciences, 2018

Cell counting Kit-8 (CCK-8) was from Dojindo Chemical Co., Japan. TUNEL assay kit
was purchased from Roche … 2.6. Measure of H292 cells' activity, invasion and apoptosis.
Cell viability was measured by cell counting Kit-8 (Dojindo, Tokyo, Japan) …

Cytoplasmic expression of epithelial cell transforming sequence 2 in lung adenocarcinoma and its implications for malignant progression

Z Kosibaty, Y Murata, Y Minami, T Dai, J Kano… - Laboratory Investigation, 2018

… After 48 h, cell proliferation was assessed using a cell counting kit-8 (DOJINDO, Kumamoto,
Japan) in accordance with the manufacturer's protocol. Absorbance was measured at
450 nm using a Microplate Reader (Bio-Rad Laboratories) …

[PDF] GALNT6 Promotes Tumorigenicity and Metastasis of Breast Cancer Cell via ß-catenin/MUC1-C Signaling Pathway

Y Mao, Y Zhang, S Fan, L Chen, L Tang, X Chen, J Lyu - International Journal of …, 2019

… The expression of GALNT6 in MDA-MB-231 was tested by Real-time
Quantitative PCR and Western Blotting. The positive clones were selected
for subsequent experiments. Cell proliferation assay Cell proliferation was …

[PDF] Highly expressed lncRNA FAL1 promotes the progression of gastric cancer by inhibiting PTEN

CH ZHU, DH XIAO, LB DAI, HG XU, YH JIANG… - European Review for …, 2018

… 1×104 cell/µL. 0, 24, 48 and 72 h after culture, 10 µL cell counting kit-8 solution
(CCK-8, Dojindo, Kumamoto, Japan) was added in each well, fol- lowed by incubation
at 37°o for 2 h in the dark. The absorbance at the wavelength …

[PDF] GAS5 promotes myocardial apoptosis in myocardial ischemia-reperfusion injury via upregulating LAS1 expression

SD LIU, WX MENG, L Xu, C Chi, X Sun, HY LIU - European Review for Medical and …, 2018

… CCK-8 (Cell Counting Kit-8) Assay Transfected cells were seeded into 96-well plates
at a density of 2×103/µL. 10 µL of CCK-8 solution (cell counting kit-8, Dojindo, Kumamoto,
Japan) was added in each well after cell culture for 0, 1, 2, and 3 days, respectively …

[HTML] Peptide-Templated Gold Clusters as Enzyme-Like Catalyst Boost Intracellular Oxidative Pressure and Induce Tumor-Specific Cell Apoptosis

Y Zhang, X Zhang, Q Yuan, W Niu, C Zhang, J Li, Z He… - Nanomaterials, 2018

… Beyotime Institute of Biotechnology (Haimen, China). Apoptosis Detection Kit (Annexin
V-FITC/PI) and cell counting kit-8 (CCK-8) were acquired from Dojindo Laboratories
(Kumamoto, Japan). ß-actin antibody, PARP antibody, anti …

[PDF] Long non-coding RNA H19 promotes proliferation of Hodgkin's lymphoma via AKT pathway

Y Wang, L Wang, M Sui - JOURNAL OF BUON, 2018

… Ten µL of CCK-8 solution (cell counting kit-8, Dojindo, Kumamoto, Japan)
were added in each well after cell culture for 6, 24, 48, 72 and 96 hrs,
respectively. The absorbance at 450 nm of each sample was measured …

Baicalin alleviates 6-hydroxydopamine-induced neurotoxicity in PC12 cells by down-regulation of microRNA-192-5p

C Kang, L Wang, M Kang, X Liu, Y Fu, J Gao - Brain Research, 2018

… 4.2. Cell Counting Kit-8 (CCK-8) assay. CCK-8 (Dojindo Molecular Technologies,
Gaithersburg, MD) was used for cell viability. Briefly, inoculate cell suspension around
100 µL/well in a 96-well plate at a density of 5000 cells/well …

Trichosanthin inhibits the proliferation of cervical cancer cells and downregulates STAT-5/C-myc signaling pathway

Y Chen, L Han, L Bai, H Tang, Z Ai - Pathology-Research and Practice, 2018

… 2.3. Cell viability assay. Cell viability was measured by Cell Counting Kit-8 (CCK-8)
(Dojindo, Japan) following manuscript protocol. Briefly, HeLa and caski cells were plated
in 96-well plates at a density of 5 × 10 3 cells/well and cultured overnight …

Long noncoding RNA H19 promotes vascular remodeling by sponging let-7a to upregulate the expression of cyclin D1

W Sun, J Lv, L Duan, R Lin, Y Li, S Li, C Fu, L Zhao… - … and Biophysical Research …, 2018

… plate. 18 h later, the virus solution was removed and replaced with normal medium.
2.5. Cell-counting kit-8 (CCK-8) assay. The proliferation of T/G HA-VSMC cells was
determined using the CCK-8 kit (Dojindo, Japan). Cells …

[HTML] Curcumin inhibits proliferation, migration, invasion and promotes apoptosis of retinoblastoma cell lines through modulation of miR-99a and JAK/STAT pathway

Y Li, W Sun, N Han, Y Zou, D Yin - BMC Cancer, 2018

… Thereafter, 0–50 µM of curcumin was added to treat cells for 24 h. Cell
Counting Kit-8 (CCK-8, Dojindo Molecular Technologies, Gaithersburg,
MD) solution (10 µL) was then added, and the cultures were incubated at …

Effect of gap junction-mediated intercellular communication on TGF-ß induced epithelial-to-mesenchymal transition

S Fukuda, M Akiyama, H Harada, K Nakahama - Biochemical and Biophysical …, 2018

… The cell proliferation was measured at day3, 5 and 7 using Cell Counting
Kit-8 (Dojindo, Japan), according to the manufacturer's protocol, followed
by colorimetric detection at 450 nm using ARVO MX 1420 multilabel counter …

Activation of Keap1-Nrf2 signaling by 4-octyl itaconate protects human umbilical vein endothelial cells from high glucose

C Tang, S Tan, Y Zhang, L Dong, Y Xu - Biochemical and Biophysical Research …, 2018

… Its value was normalized to the control. 2.7. Viability assay. HUVECs were
originally seeded into the 96-well plates (at 2.5 × 10 4 cells/cm 2 ). Following
the treatments, cell viability was measured by Cell Counting Kit-8

[PDF] Preconditioning of umbilical cord-derived mesenchymal stem cells by rapamycin increases cell migration and ameliorates liver ischaemia/reperfusion injury in mice …

J Zheng, H Li, L He, Y Huang, J Cai, L Chen, C Zhou… - Cell Proliferation, 2018

… After treatment for 12, 24 and 48 hours, a Cell Counting Kit-8 (CCK-8; Dojindo, Kumamoto,
Japan) was used according to the manufacturer's in- structions. An Annexin V-propidium
iodide apoptosis detection kit (Kaiji Bio-Technology Co …

Piceatannol-3-O-ß-D-glucopyranoside (PG) exhibits in vitro anti-metastatic and anti-angiogenic activities in HT1080 malignant fibrosarcoma cells

A Kim, JY Ma - Phytomedicine, 2018

… µM PG for 48 hours. Cell viability was measured using the Cell Counting Kit-8
(CCK-8; Dojindo Molecular Technologies, Inc., Kumamoto, Japan) according to
the manufacturer's protocol. Absorbance at 540 nm was detected …

Circular RNA circ_0034642 elevates BATF3 expression and promotes cell proliferation and invasion through miR-1205 in glioma

M Yang, G Li, L Fan, G Zhang, J Xu, J Zhang - Biochemical and Biophysical Research …, 2018

… 2.4. Cell proliferation assays. Cell proliferation was measured by Cell Counting
Kit-8 (CCK-8; Dojindo Molecular Technologies, USA). In short, glioma cells were
cultured in 96-well plates (2 × 10 3 cells/well). 10 µL of CCK-8 …

MicroRNA-6807-3p promotes the tumorigenesis of glioma by targeting downstream DACH1

GF Lu, F Geng, Z Xiao, YS Chen, Y Han, CY You… - Brain Research, 2018

… Transfection cells were harvested for 48h before further analysis. 4.5.
Cell Counting Kit-8 (CCK-8) assay. Cell viability was monitored using
a WST-8 Cell Counting Kit-8 (CCK-8; Dojindo, Tokyo, Japan), and …

[HTML]Epigenetic silencing of Lgr5 induces senescence of intestinal epithelial organoids during the process of aging

R Uchida, Y Saito, K Nogami, Y Kajiyama, Y Suzuki… - npj Aging and Mechanisms …, 2018

Article.

[PDF]Zhongfenggao protects brain microvascular endothelial cells from oxygen-glucose deprivation/reoxygenation-induced injury by angiogenesis

S Huang, T Gong, T Zhang, X Wang, Q Cheng, Y Li - Biological and Pharmaceutical …, 2018

… Page 6. Biological and Pharmaceutical Bulletin Advance Publication purchased from
Beyotime Institute of Biotechnology (Jiangsu, China). The cell counting kit-8 (CCK-8) was
provided by Dojindo (Kumamoto, Japan). DAPT was purchased from Selleck …

[PDF]K6PC-5 Activates SphK1-Nrf2 Signaling to Protect Neuronal Cells from Oxygen Glucose Deprivation/Re-Oxygenation

H Liu, Z Zhang, M Xu, R Xu, Z Wang, G Di - Cellular Physiology and Biochemistry, 2018

… Cell survival, apoptosis and death assay. The Cell Counting Kit-8 (CCK-8) assay (Dojindo
Laboratories, Kumamoto, Japan) was performed to examine the viability of neuronal
cells. The CCK optical density (OD) at 450 nm was recorded …

[HTML]Sox2 is associated with cancer stem-like properties in colorectal cancer

K Takeda, T Mizushima, Y Yokoyama, H Hirose, X Wu… - Scientific Reports, 2018

Article.

Expression of Genes Involved in the Non-Neuronal Cholinergic System and Their Possible Functions during Ovarian Follicular Development in Mice

M Ueno, H Ito, T Kanke, Y Matsuno, W Fujii, K Naito… - Journal of Mammalian Ova …, 2018

… After the culture, cell proliferation assays were performed using Cell Counting
Kit-8 (Dojindo, Tokyo, Japan) according to the manufacturer's instruction. Cultures
were conducted at 37 °C in 5% O 2 , 5% CO 2 , and 90% N 2 …

[HTML]Variant of SNP rs1317082 at CCSlnc362 (RP11-362K14. 5) creates a binding site for miR-4658 and diminishes the susceptibility to CRC

C Shen, T Yan, Z Wang, H Su, X Zhu, X Tian, JY Fang… - Cell Death & Disease, 2018

Cell Counting Kit-8 (Dojindo Molecular Technologies, Japan) was used to incubate with
cells away from light for 2 h at 37 °C at the time points of 24, 48, 72, 96, and 120 h after
plantation. The absorbance was measured with a wavelength 450 nm (450 OD) …

[PDF]miR-10b Downregulated by DNA Methylation Acts as a Tumor Suppressor in HPV-Positive Cervical Cancer via Targeting Tiam1

M Yu, Y Xu, L Pan, Y Feng, K Luo, Q Mu, G Luo - Cellular Physiology and …, 2018

… Cell proliferation assay. Cell proliferation was measured using a Cell Counting Kit-8
(CCK-8) (Dojindo, Japan) according to the manufacturer's instructions. Briefly, cells were
seeded into a 96-well plate (5×10 3 cells/well), with six replicate wells per group …

[HTML]Tetraspanin 1 promotes epithelial-to-mesenchymal transition and metastasis of cholangiocarcinoma via PI3K/AKT signaling

Y Wang, Y Liang, G Yang, Y Lan, J Han, J Wang, D Yin… - Journal of Experimental & …, 2018

… with 5% CO 2 to allow attachment. Cell viability at various time points was measured
using Cell Counting Kit-8 (CCK-8) assays (CK04–01; Dojindo Molecular
Technologies, Inc., Japan). The primary medium in the wells was …

[PDF]SIRT7 Regulates the Vascular Smooth Muscle Cells Proliferation and Migration via Wnt/ß-Catenin Signaling Pathway

J Zheng, K Chen, H Wang, Z Chen, Y Xi, H Yin, K Lai… - BioMed Research …, 2018

… Wnt/ß-catenin pathway, was used in SIRT7 overexpression HAVSMCs;
subsequently cells proliferation and migration were assessed by Cell Counting
Kit-8 assay, flow … and incubated at 37°C for 6 h. The cell viability was examined …

Polymeric micelles deliver phthalocyanine for enhancement in vitro photodynamic efficacy

K Chen, R Guo, S Xiao, Q Ye, H Yang, Y Peng - Advanced Optical Imaging …, 2018

… purchased from Sigma-Aldrich were used as received without further purification.
Cell counting kit-8 (CCK-8) assay was obtained from Dojindo Laboratories (Japan).
IR spectrum was measured on a Perkin Elmer IR spectrometer …

Up-regulation of long noncoding RNA MINCR promotes non-small cell of lung cancer growth by negatively regulating miR-126/SLC7A5 axis

J Wang, M Ding, H Zhu, Y Cao, W Zhao - Biochemical and Biophysical Research …, 2018

… 2.4. Cell proliferation assay. Cell proliferation was performed with the Cell Counting
Kit-8 (Dojindo, Japan). The transfected cells were seeded in 96-well plates and
cultured in complete medium for 0, 24, 48, 72 and 96 h. After …

[HTML]miR-34a Inhibits Cell Proliferation by Targeting SATB2 in Hepatocellular Carcinoma

G Wu, Z Li, Y Wang, X Ju, R Huang - BioMed Research International, 2018

… 2.5. Cell Viability by CCK-8 Assay. Cell proliferation was evaluated using a Cell Counting
Kit-8 (CCK-8; Dojindo Molecular Technologies, Inc., Kumamoto, Japan). HepG2 cells
(5×10 3 / well) were suspended and cultured in 96-well plates overnight …

DNA methylation regulates Microtubule-associated tumor suppressor 1 in human non-small cell lung carcinoma

S Parbin, N Pradhan, L Das, P Saha, M Deb… - Experimental Cell Research, 2018

… Cells were counted in triplicate on each day for five days using the Cell Counting Kit-8
(CK04–11; Dojindo, Rockville, MD). 2.11. Colony formation assay. Cells were seeded
at low density cells (600 per well) in 35 mm petri-plates and allowed to attach for 24 hours …

Cordycepin sensitizes breast cancer cells toward irradiation through elevating ROS production involving Nrf2

J Dong, Y Li, H Xiao, D Luo, S Zhang, C Zhu, M Jiang… - Toxicology and Applied …, 2018

… 2.3. CCK-8 assay. The effects of cordycepin and irradiation in normal cells were
evaluated by cell counting assay using a Cell Counting Kit-8 (CCK-8) (Dojindo
Laboratories, Kumamoto, Japan). Cells were seeded in 96-well …

Targeting Mcl-1 inhibits survival and self-renewal of hepatocellular cancer stem-like cells

H Zhang, G Li, G Chen, Y Zhang, J Pan, H Tang, J Li… - Clinics and Research in …, 2018

… Cell viability assays. Cells were seeded in triplicate, 5,000/well, in a 96-well plate
and treated as indicated in text for 48 h. Cell viability was examined by Cell Counting
Kit-8 (CCK-8) assay (Dojindo, Shanghai, China). Results …

Hsa-miRNA-29a protects against high glucose-induced damage in human umbilical vein endothelial cells

Z Huang, N Li, Y Shan, C Liang - Journal of Cellular Biochemistry, 2018

… Seventy-two hours after viral infection, the cells were reseeded, cultured
in NG or HG for 72 hours, and subjected to cell counting kit-8 (CCK-8)
assay for proliferation. Briefly, 10 µL CCK-8 solution (Dojindo, Japan) …

Inhibition of dengue virus by curcuminoids

A Balasubramanian, R Pilankatta, T Teramoto… - Antiviral Research, 2018

… 2.5. Cell-based cytotoxicity assay. The viability of cells in the presence of
varying concentration of curcuminoids was measured using the Cell Counting
Kit-8 (Dojindo, Rockville, MD) and the concentration at which the cell viability …

[PDF]MALAT1 promotes cisplatin resistance in cervical cancer by activating the PI3K/AKT pathway

N Wang, MS HOU, Y Zhan, XB SHEN, HY XUE - European Review for Medical and …, 2018

Cell Counting Kit-8 (CCK-8) Assay HeLa and C-33A cells in logarithmic growth
phase were collected and seeded in 96-well plates at a density of 3 … After
incubation for another 24 h, a total of 10 µL of CCK-8 (Dojindo, Kumamoto …

[PDF]MicroRNA-495 inhibits the progression of non-small-cell lung cancer by targeting TCF4 and inactivating Wnt/ß-catenin pathway

HE ZHENG, G Wang, J Song, Y Liu, YM LI, WP DU - European Review for Medical …, 2018

Cell Counting Kit-8 (CCK-8) Assay CCK-8 assay was performed to measure
cell proliferation based on the manufacturer's in- structions … in an incubator
with 5% CO2 at 37°C. Next, 10 µL CCK-8 reagents were added in each …

DJ-1 Alters Epirubicin-induced Apoptosis via Modulating Epirubicinactivated Autophagy in Human Gastric Cancer Cells

X Pan, F Su, L Xu, Z Yang, D Wang, L Yang, F Kong… - Current Medical Science, 2018

… 1.3 CCK-8 Assay The effects of epirubicin or DJ-1 knockdown/ overexpression on tumor
cell viability were measured with Cell Counting Kit-8 (CCK-8) reagent (Dojindo Kumamoto,
Japan). Cells were seeded on 96-well plates (1000 cells per well) in complete RPMI-1640 …

[HTML]Dual-targeting and microenvironment-responsive micelles as a gene delivery system to improve the sensitivity of glioma to radiotherapy

X Jiao, Y Yu, J Meng, M He, CJ Zhang, W Geng, B Ding… - Acta Pharmaceutica Sinica …, 2018

… YOYO-1 iodide was purchased from Thermo Fisher Scientific (Eugene, OR,
USA). The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular
Technologies, Inc. (Nanjing, China). Annexin V-APC Apoptosis Analysis …

Long noncoding RNA CASC9 promotes LIN7A expression via miR-758-3p to facilitate the malignancy of ovarian cancer

X Hu, Y Li, D Kong, L Hu, D Liu, J Wu - Journal of Cellular Physiology

… Then proliferation was determined through the cell counting kit-8 (CCK8, Dojindo, Tokyo,
Japan) according to the manufacturer's protocol. 2.5 Colony formation assay. One thousand
cells per well were seeded into the six-well plates and cultured for 14 days …

Mutual inhibitions between epidermal growth factor receptor signaling and miR-124a control pancreatic progenitor proliferation

Z Zhang, W Zhai, J Liang, Z Chen, M Ma, Y Zhao… - Journal of Cellular Physiology

… Cells proliferation was evaluated by the Cell Counting Kit-8 (CCK8) assay 48 hr after
treatment … 2.5 CCK8 assay and cell counting. Cell numbers were assayed by the CCK8
(Dojindo Laboratories, Kumamoto, Japan) and cell counting …

LncRNA MALAT1 protects cardiomyocytes from isoproterenol-induced apoptosis through sponging miR-558 to enhance ULK1-mediated protective autophagy

X Guo, X Wu, Y Han, E Tian, J Cheng - Journal of Cellular Physiology

… 2.4 Cell viability assay. Cell viability assay was conducted using the Cell Counting
Kit-8 (CCK-8; Dojindo, Kumamoto, Japan). Briefly, H9C2 cells were seeded in the
96-well plated and were given different treatments for the …

Icariin inhibits RANKL-induced osteoclastogenesis via modulation of the NF-?B and MAPK signaling pathways

Q Xu, G Chen, X Liu, M Dai, B Zhang - Biochemical and Biophysical Research …, 2018

… 2. Materials and methods. 2.1. Cell viability assay. In order to evaluate the
cytotoxic effect of icariin, we used the Cell Counting Kit-8 (CCK-8, Dojindo
Molecular Technologies, Inc., Kumamoto, Japan) according to the …

[PDF]Apigenin Protects the Brain against Ischemia/Reperfusion Injury via Caveolin-1/VEGF In Vitro and In Vivo

Q Pang, Y Zhao, X Chen, K Zhao, Q Zhai, F Tu - Oxidative Medicine and Cellular …, 2018

… apigenin in glucose-free culture followed by reoxygenation and cultured in normal
conditions. Cell viability was measured by Cell Counting Kit-8 (CCK8) (Dojindo, Japan).
After treatment with 20 µL of CCK8 solution at 37°C for 2 …

[PDF]eIF3d is an independent prognostic parameter in colorectal cancer and promotes proliferation via c-Jun/cyclin D1 in CRC cell line

C Yang, B Zhao, C Li - Int J Clin Exp Med, 2018

… Cell proliferation and cell cycle assay Cell proliferation was performed with Cell
Counting Kit-8 (CCK-8) (Dojindo, Japan). According to the instructions, Cell Counting
Kit-8 reagent was added at 0, 24, 48, and 72 h respectively …

[PDF]miR-145 and miR-143 expression differ between Uygur and Han women with endometrial cancer

X Wang, W Li, D He, H Wang, R Han, J Zhao, Y Zhang… - Int J Clin Exp Med, 2018

… At specified time points, 10 µl CCK-8 (Cell Counting Kit-8) reagent (Dojindo, Tokyo, Japan)
was added to each well and then the cells were incubated at 37°C for an additional 2
hours. The absorbance was measured at 450 nm. Cell migration and invasion assays …

[PDF]Huiyang Shengji Extract improved wound healing by regulating macrophage phenotype transition

X He, Y Lin, Y Meng, Y Xue, X Han, P Li - Int J Clin Exp Med, 2018

… The macrophages were then incubated in fresh RPMI 1640 (10% FCS, 1%
L-glutamine). The activity of the macro- phages was tested with Cell Counting
kit-8 (Dojindo Molecular Technologies Inc., Japan), and the phagocytic …

[PDF]Under-expression of LKB1 is associated with enhanced p38-MAPK signaling in human hepatocellular carcinoma

L Sha, F Lian, K Li, C Chen, Y Zhao, J He, S Huang… - Int J Clin Exp Pathol, 2018

… tion experiments. For the cell proliferation assay, the Cell Counting Kit-8 (CCK-8)
(Dojindo, Kumamoto, Japan) was used. After 24 hours of transfection, the cells were
collected by cen- trifugation and resuspended. The cells …

[PDF]Emodin inhibits formation of hypertrophic scars through regulating the p38/MAPK signaling pathway

B He, J Chen, L Liu, H Wang, S Wang, P Li, J Zhou - Int J Clin Exp Med, 2018

… Louis, MO, USA) at 0.1 mg/ml for 4 hours at 37°C on a rotator to isolate fibroblasts (HSFs).
HSFs were (Dojindo Laboratories, Kumamoto, Japan) … Cell proliferation assay Cell
proliferation assay was perform- ed using cell counting kit-8 (CCK-8) Page 3 …

[PDF]IRS-1 regulates proliferation, invasion and metastasis of pancreatic cancer cells through MAPK and PI3K signaling pathways

Y Huang, L Zhou, X Meng, B Yu, H Wang, Y Yang… - Int J Clin Exp Pathol, 2018

… The cells were seeded at a density of 5000 cells per well in 96-well plates
in culture medi- um containing 10% FBS. After 3 days, the num- ber of viable
cells was counted using the Cell Counting Kit-8 (Dojindo Co., Kumamoto …

INSULIN-LIKE GROWTH FACTOR-CHEMOTHERAPEPUTIC CONJUGATE FOR TREATING MYELODYSPLASTIC SYNDROME

H Mctavish, AZ Dudek - US Patent App. 16/006,561, 2018

… Day 5. Cell proliferation is determined by adding Dojindo CCK-8 reagent and incubating
and measuring absorbance of the dye according to the manufacturer's instructions. Result:
Results of a representative cytotoxicity assay with 765IGF-MTX are shown in FIG …

E4F1 silencing inhibits the cell growth through cell-cycle arrest in malignant transformed cells induced by hydroquinone

Q Tan, J Li, J Peng, Z Liu, J Liu, H Zhang, Q Yuan… - Journal of Biochemical and …

… 2.2 Cell proliferation assay. Changes in cell proliferation were assessed
using a cell counting kit-8 (CCK-8) kit (Dojindo, Tokyo, Japan). In a
96-well plate, 1000 cells in 100 µL serum-free Roswell Park Memorial …

[PDF]Alsterpaullone induces apoptosis of HepG2 cells via a p38 mitogen-activated protein kinase signaling pathway

P Yin, N Zheng, J Dong, C Xu, X Zhang, G Ding - Oncology Letters, 2019

… was consistently <0.1%. A Cell Counting kit-8 (CCK-8) was purchased
from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). Dulbecco's
modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were …

[PDF]Long noncoding RNA LINC00460 promotes carcinogenesis via sponging miR-613 in papillary thyroid carcinoma

L Feng, B Yang, XD Tang - Journal of Cellular Physiology, 2018

… 2.5 | Cell proliferation assay Cell proliferation was detected by Cell
Counting Kit-8 (CCK-8; Dojindo, Tokyo, Japan). Briefly, transfected cells
were grown in TABLE 1 Correlation between clinicopathological features …

CircVRK1 regulates tumor progression and radioresistance in esophageal squamous cell carcinoma by regulating miR-624-3p/PTEN/PI3K/AKT signaling pathway

Y He, E Mingyan, C Wang, G Liu, M Shi, S Liu - International Journal of Biological …, 2018

… The cells were transfected with oeCircRNA or Vector, miR-624-3p inhibitor or miR-NC
for 24 h and then incubated at 37 °C. Afterwards, Cell Counting kit-8 solution (CCK-8,
Dojindo, Mashikimachi, Japan) was added to the ESCC cells for incubation …

Long non-coding RNA RUNX1-IT1 plays a tumour-suppressive role in colorectal cancer by inhibiting cell proliferation and migration

J Shi, X Zhong, Y Song, Z Wu, P Gao, J Zhao, J Sun… - Cell Biochemistry and …, 2018

… 2.6 | Cell proliferation assay The potential for cell proliferation was determined using the
cell counting kit 8 (CCK-8, Dojindo). Approximately 3 × 103 cultured HCT116 cells or
RKO cells were seeded in the 96-well plates for 24, 48, 72, and 96 hours …

MicroRNA-181a-5p suppresses cell proliferation by targeting Egr1 and inhibiting Egr1/TGF-ß/Smad pathway in hepatocellular carcinoma

J Bi, J Zheng, Q Li, S Bao, X Yu, P Xu, C Liao - … Journal of Biochemistry & Cell Biology, 2018

… The experiments were conducted in triplicate. 2.7. Cell counting kit-8 assay. The
proliferation of HCC cells were measured by CCK-8 (Dojindo, Kumamoto, Japan)
according to the manufacturer's protocols. The absorbance was measured at 450 nm …

Folate-conjugated, mesoporous silica functionalized boron nitride nanospheres for targeted delivery of doxorubicin

S Feng, H Zhang, S Xu, C Zhi, H Nakanishi, XD Gao - Materials Science and …, 2018

… FBS) were obtained from Gibco (NY, USA). Cell Counting Kit-8 (CCK-8) was acquired
from Dojindo (Kumamoto, Japan), LIVE/DEAD Viability/Cytotoxicity Kit was bought
from Invitrogen (Carlsbad, CA). 2.2. Synthesis of BNMS …

Triptonide inhibits human nasopharyngeal carcinoma cell growth via disrupting Lnc-RNA THOR-IGF2BP1 signaling

S Wang, Y Lv, XC Xu, Y Zuo, Y Song, G Wu, PH Lu… - Cancer Letters, 2018

Cell Counting Kit-8 (CCK-8, Dojindo, Japan) was employed to evaluate cell viability
according to the manufacturer's instructions. CCK-8 optical density (OD) at 450 nm was
measured under a microplate reader (Bio-Rad Laboratories). Colony forming assay …

Isolinderalactone regulates the BCL-2/caspase-3/PARP pathway and suppresses tumor growth in a human glioblastoma multiforme xenograft mouse model

JY Hwang, JH Park, MJ Kim, WJ Kim, KT Ha, BT Choi… - Cancer Letters, 2018

… 2.2. Cell Counting Kit -8 assay. Cell viability was evaluated using the Cell Counting
Kit-8 (CCK-8; Dojindo, Kumamoto, Japan) assay according to the manufacturer's
instructions. U-87 GBM cells were seeded on a 96-well plate …

LincRNA-p21 enhances the sensitivity of radiotherapy for gastric cancer by targeting the ß-catenin signaling pathway

L Chen, D Yuan, Y Yang, M Ren - Journal of Cellular Biochemistry, 2018

… Data are expressed as mean ± SD from three independent experiments. 2.4 Cell
proliferation, cell cycle, and migration assay. Cell proliferation was monitored using Cell
Counting Kit-8 (CCK-8) analysis (Dojindo, Kumamoto, Japan) following the guidelines …

[HTML]Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/CBX8 …

Y Xu, Y Yao, K Leng, D Ji, L Qu, Y Liu, Y Cui - Cellular Physiology and Biochemistry, 2018

… Cell counting kit detection. Cell counting kit-8 (CCK-8, Dojindo, Japan)
was induced to detect the cell viability. The cell suspension was poured
into the 96-well plates and maintained in 5% CO 2 at 37°C. 10µL of …

[HTML]Astragaloside protects oxygen and glucose deprivation induced injury by regulation of microRNA-21 in retinal ganglion cell line RGC-5

H Bao, D Sun, P Qi, S Jiang - Biomedicine & Pharmacotherapy, 2019

… The detail experiment steps were following the manufacturer's protocol. 2.3. Cell viability
assay. The viability of RGC-5 cells was measured by Cell Counting Kit-8 (CCK-8,
Dojindo Molecular Technologies, Gaithersburg, MD) assay …

[HTML]Iron removal enhances vitamin C-induced apoptosis and growth inhibition of K-562 leukemic cells

M Tsuma-Kaneko, M Sawanobori, S Kawakami, T Uno… - Scientific Reports, 2018

Article.

[PDF]SLC25A22 Promotes Proliferation and Metastasis of Osteosarcoma Cells via the PTEN Signaling Pathway

MW Chen, X Wu - Technology in Cancer Research & Treatment, 2018

Osteosarcoma is a highly malignant bone tumor. However, due to the high complexity
of the occurrence and metastasis of osteosarcoma, the exact mechanism promoti...

Long Stress Induced Non-Coding Transcripts 5 (LSINCT5) Promotes Hepatocellular Carcinoma Progression Through Interaction with High-Mobility Group AT-hook 2 …

O Li, Z Li, Q Tang, Y Li, S Yuan, Y Shen, Z Zhang, N Li… - Medical Science Monitor, 2018

… Page 3. Viability assay The Cell Counting Kit-8 (CCK-8; Dojindo Molecular Technologies,
Inc., Kumamoto, Japan) was used to measure the viability. Indicated cells were
re-suspended and seeded into a 12-well plate (~105 cells/well) for 5 days …

[PDF]Tryptophan Photoproduct FICZ Upregulates IL1A, IL1B, and IL6 Expression via Oxidative Stress in Keratinocytes

Y Tanaka, H Uchi, A Hashimoto-Hachiya, M Furue - Oxidative Medicine and Cellular …, 2018

… 2.3. Cell Viability. The viability of HaCaT cells was determined by using a Cell
Counting Kit-8 (Dojindo Molecular Technologies, Inc., Kumamoto, Japan) in
accordance with the manufacturer's instructions. 2.4. Flow Cytometry …

[HTML]Novel Calcium Phosphate Cement with Metformin-Loaded Chitosan for Odontogenic Differentiation of Human Dental Pulp Cells

W Qin, JY Chen, J Guo, T Ma, MD Weir, D Guo, Y Shu… - Stem Cells International, 2018

… The percentage of live cells and the live cell density were calculated as previously
described [21]. 2.6. Cell Proliferation Assays. Cell proliferation was measured by
the Cell Counting Kit-8 assay (CCK-8, Dojindo, Tokyo, Japan) …

[HTML]EBV-miR-BART8-3p induces epithelial-mesenchymal transition and promotes metastasis of nasopharyngeal carcinoma cells through activating NF-?B and Erk1/2 …

C Lin, J Zong, W Lin, M Wang, Y Xu, R Zhou, S Lin… - Journal of Experimental & …, 2018

Epstein-Barr virus (EBV) is ubiquitously associated with nasopharyngeal
carcinoma (NPC). EBV encodes two groups of microRNAs (miRNAs) which
are divided into BamHI fragment H rightward open reading frame 1 (BHRF1) …

miR-141 alleviates LPS-induced inflammation injury in WI-38 fibroblasts by up-regulation of NOX2

B Quan, H Zhang, R Xue - Life Sciences, 2018

… Cell viability was determined using Cell Counting Kit-8 (CCK-8) solution
(Beyotime, Shanghai, China) … well) at 37 °C in a humidified atmosphere with
5% CO 2 . After incubation of 24 h, cells were treated by Annexin …

[PDF]TIMP-1 inhibits proliferation and osteogenic differentiation of hBMSCs through Wnt/ß-catenin signaling

T Liang, W Gao, L Zhu, J Ren, H Yao, K Wang, D Shi - Bioscience Reports, 2018

… Proliferation assay hBMSCs proliferation was determined by 2-(2-methoxy-
4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfo-phenyl) -2H-tetrazolium,
monosodium salt (WST-8) assay (Cell Counting Kit-8, CCK-8, Dojindo, Japan) …

Fabrication of perfusable 3D hepatic lobule-like constructs through assembly of multiple cell type laden hydrogel microstructures

J Cui, H Wang, Z Zheng, Q Shi, T Sun, Q Huang… - Biofabrication, 2018

… Quantitative analysis of cell proliferation was performed using a Cell Counting Kit-8
(CCK-8) assay (Dojindo Molecular Technologies Inc., Kumamonto, Kyushu, Japan). The
absorbance of the samples was measured using a microplate reader (Molecular Devices) …