MSDS

Chemical Name: 3',6'-Di(O-thiosalicyl)fluorescein

Appearance: White to pale yellow powder or solid
Purity: ≧ 90.0 %
MW: 604.65

Storage Condition: 0-20°C, protect from light
Shipping Condition: Ambient temperature

Product Description:

It becomes obvious that there are a lots of molecules containing sulfane sulfurs such as persulfides and polysulfides in living organism. These molecular species are involved in production, storage and release of hydrogen sulfide, which is recognized as an important physiological mediator. Furthermore, recent studies reveal that persulfides and polysulfides may control intracellular signal transduction through s-sulfhydration of proteins.
SSP4 (Sulfane Sulfur Probe 4) is a novel fluorescent probe to detect sulfane sulfurs selectively. SSP4 itself is non-fluorescent, but it emits strong green fluorescence when it reacts with sulfane sulfurs. Thus, SSP4 enables high sensitive fluorescence detection and imaging of sulfane sulfurs.

Fig. 1 Chemical Reaction with sulfane sulfer Fig. 2 Fluorescence spectrum (Em: 482nm/Ex: 515nm)

Chemical species containing sulfane sulfurs:

Fig. 3 The formation of sulfane sulfer in vivo changes due to reduction/oxidation and translation


References:

1) W. Chen, C. Liu, B. Peng, Y. Zhao, A. Pacheco, and M. Xian,“New fluorescent probe for sulfane sulfurs and the application in bioimaging E Chem. Sci., 2013, 4, 2892.
2) T. Ida, T. Sawa, H. Ihara. Y. Tsuchiya, Y. Watanabe, Y. Kumagai, M. Suematsu, H. Motohashi, S. Fujii, T, Matsunaga, M. Yamamoto, K. Ono, N. O. Devarie-Baez, M. Xian, J. M Fukuto, and T. Akaike, “Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling E Proc Natl Acad Sci U S A., 2014, 111, 7606.
3) E. Marutani, M. Sakaguchi, W. Chen, K. Sasakura, J. Liu, M. Xian, K. Hanaoka, T. Nagano, and F. Ichinose, "Cytoprotective effects of hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonists mediated by intracellular sulfane sulfur", Med. Chem. Commun., 2014, 5, 1577.
4) M. Sakaguchi, E. Marutani, H-S. Shin, W. Chen, K. Hanaoka, M. Xian, and F. Ichinose, “Sodium Thiosulfate Attenuates Acute Lung Injury in Mice E Anesthesiology. 2014,121, 1248.


Cell Imaging:








Cell: CHO cell
Exposure time: 1,000 msc

Left: Control cells
Right: Treatment of 100 μmol/l Na2S3

Item # Description/Size Availability Qty Break Price Quantity
SB10-10
SSP4 (1 mg)
1-2 business days 1 $290.00

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