Storage Condition: 0-5°C
Shipping Condition: Ambient temperature
Protein thiol is a major redox signaling in the
cellular processes. Modification of protein thiol by oxidative stress associate
with the redox state in the cells. The approach of this kit is detecting the
number of thiol residues accurately in order to study the redox state in oxidative
Fig. 1 Redox States of Thiol group(s) in Protein
Mechanism of Thiol Modification with Protein-SHifter:
Fig. 2 Maleimido group of the Protein-SHifter
binds to a free thiol group of a protein. The conjugate consists of a unique
molecular weight that shifts properly in electrophoresis.
Photocleavage method enhances membrane transcription efficiency in western blotting.
Visualization of Redox State of Thiol Residues in a Protein:
Fig. 3 One molecule of Protein-SHifter binds to one molecule of free-thiol in protein. The number of the conjugation is directly proportional to the number of free thiol as clear band-shift.
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Detection of the number of thiol on the GAPDH in HeLa cell lysate: