• Simple preparation of Biotin-SAM on gold surface
  • Efficient Immobilization of Avidins
  • Suppress unspecific binding with unknown proteins
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    Appearance: White or slightly yellowish solid

    Storage Condition: 0-5°C
    Shipping Condition: Blue Ice

    Keyword:
    Self-Assembled Monolayers, SAM, SPR, QCM, biosensors, protein immobilization

    Product Description
    There are three ways to immobilize a protein on a Surface Plasmon Resonance (SPR) or Quartz Crystal Microbalance (QCM) biosensor consisting of Self-Assembled Monolayer (SAM); 1) Formation of covalent bond of amine functions on proteins and activated carboxy-SAM; 2) immobilize proteins by His-Tag through Ni-NTA-terminated SAM; 3) Immobilization of proteins through Biotin and Avidin interaction. Because the Biotin-Avidin formation is known as a facile reaction, it is widely utilized for immobilization of protein (Fig 1).

                                                        Fig.1 A biosensor construction by Biotin-SAM Formation Reagent





    Fig. 2  Comparison of the amount of bound streptavidin, unspecific binding of FBS, bound biotinylated BSA between Biotin-SAM Formation Reagent and Company A.

    Item # Description/Size Availability Qty Break Price Quantity
    B564-10
    1 µmol x 3
    1-2 business days 1 $213.00

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