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SAM Formation Reagent
Biotin-SAM Formation Reagent
Simple preparation of Biotin-SAM on gold surface
Efficient Immobilization of Avidins
Suppress unspecific binding with unknown proteins
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White or slightly yellowish solid
Self-Assembled Monolayers, SAM, SPR, QCM, biosensors, protein immobilization
There are three ways to immobilize a protein on a Surface Plasmon Resonance (SPR) or Quartz Crystal Microbalance (QCM) biosensor consisting of Self-Assembled Monolayer (SAM); 1) Formation of covalent bond of amine functions on proteins and activated carboxy-SAM; 2) immobilize proteins by His-Tag through Ni-NTA-terminated SAM; 3) Immobilization of proteins through Biotin and Avidin interaction. Because the Biotin-Avidin formation is known as a facile reaction, it is widely utilized for immobilization of protein (Fig 1).
A biosensor construction by Biotin-SAM Formation Reagent
Comparison of the amount of bound streptavidin, unspecific binding of FBS, bound biotinylated BSA between Biotin-SAM Formation Reagent and Company A.
1 µmol x 3
1-2 business days
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