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Home
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Surface Chemistry
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SAM Formation Reagent
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Biotin-SAM Formation Reagent
Simple preparation of Biotin-SAM on gold surface
Efficient Immobilization of Avidins
Suppress unspecific binding with unknown proteins
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Description
Manual
Appearance:
White or slightly yellowish solid
Storage Condition:
0-5°C
Shipping Condition:
Blue Ice
Keyword:
Self-Assembled Monolayers, SAM, SPR, QCM, biosensors, protein immobilization
Product Description
There are three ways to immobilize a protein on a Surface Plasmon Resonance (SPR) or Quartz Crystal Microbalance (QCM) biosensor consisting of Self-Assembled Monolayer (SAM); 1) Formation of covalent bond of amine functions on proteins and activated carboxy-SAM; 2) immobilize proteins by His-Tag through Ni-NTA-terminated SAM; 3) Immobilization of proteins through Biotin and Avidin interaction. Because the Biotin-Avidin formation is known as a facile reaction, it is widely utilized for immobilization of protein (Fig 1).
Fig.1
A biosensor construction by Biotin-SAM Formation Reagent
Fig. 2
Comparison of the amount of bound streptavidin, unspecific binding of FBS, bound biotinylated BSA between Biotin-SAM Formation Reagent and Company A.
Item #
Description/Size
Availability
Qty Break
Price
Quantity
B564-10
1 µmol x 3
1-2 business days
1
$219.00
*Your estimated shipping date is calculated based on production, payment method, destination, and your shipping option.
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