Biotin-SAM Formation Reagent

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Simple preparation of Biotin-SAM on gold surface

Efficient Immobilization of Avidins

Suppress unspecific binding with unknown proteins

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Description
Manual

Appearance: White or slightly yellowish solid

Storage Condition: 0-5°C
Shipping Condition: Blue Ice

Keyword: Self-Assembled Monolayers, SAM, SPR, QCM, biosensors, protein immobilization

Product Description
There are three ways to immobilize a protein on a Surface Plasmon Resonance (SPR) or Quartz Crystal Microbalance (QCM) biosensor consisting of Self-Assembled Monolayer (SAM); 1) Formation of covalent bond of amine functions on proteins and activated carboxy-SAM; 2) immobilize proteins by His-Tag through Ni-NTA-terminated SAM; 3) Immobilization of proteins through Biotin and Avidin interaction. Because the Biotin-Avidin formation is known as a facile reaction, it is widely utilized for immobilization of protein (Fig 1).

Fig.1 A biosensor construction by Biotin-SAM Formation Reagent

Fig. 2 Comparison of the amount of bound streptavidin, unspecific binding of FBS, bound biotinylated BSA between Biotin-SAM Formation Reagent and Company A.

Item #	Description/Size	Availability	Qty Break	Price	Quantity
B564-10	1 µmol x 3	1-2 business days	1	$226.00

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