Chemical Name: N-[5-(4-Isothiocyanatobenzyl)amido-1-carboxypentyl]iminodiacetic acid
Appearance: White or slightly yellow powder
Purity: ≥ 90.0% (HPLC)
MW: 437.47, C19H23N3O7S
Storage Condition: -20°C
Shipping Condition: with blue ice
Isothiocyanobenzyl-NTA is utilized to modify the surfaces on which amine groups are attached. Through the NTA moiety attached on the surface, genetically expressed protein, bearing a hexahistidine extension at its terminus, can be immobilized via Ni (II) (His-Tag method). Using this technique, Dr. Noji and co-workers were able to directly abserve the rotation of F1-ATPase with a fluorescence microscope.