ChemicalName: (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid, potassium salt
Appearance: Pale yellow or yellow powder
Purity: ≥98.0% (HPLC)
MW: 318.42, C11H7N2O3S2K
Storage Condition: -20ºC, Protect from light and moisture, under nitrogen gas
Shipping Condition: ambient temperature
D-Luciferin is a firefly luciferase substrate. Its quantum efficiency is 0.88, which is 20 times that of Luminol. The reaction mechanism is shown below. First, Luciferin reacts with ATP by luciferase in the presence of magnesium ion, then it is oxidized to form dioxetane structure and emits yellow-green light. Luciferin-luciferase luminescence is used for ATP monitoring to determine cell viability and bacteria counting. It is also used for reporter gene assays.
1. V. T. Nguyen, et al., Firefly Luciferase Luminescence Assays Using Scintillation Counters for Quantitation in Transfected Mammalian Cells. Anal Biochem. 1988;171:404-408.
2. S. P. Crouch, et al., The Use of ATP Bioluminescence as a Measure of Cell Proliferation and Cytotoxicity. J Immunol Methods. 1993;160:81-88.
3. S. R. Ford, et al., Improvements in the Application of Firefly Luciferase Assays. Methods Mol Biol. 1998;102:3-20.