Chemical Name: 8-Amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N',N'-tetraacetic acid, tetrapotassium salt
Appearance: pale yellow powder
Purity: ≥95.0 %(HPLC)
MW: 693.87, C26H23K4N3O10
Storage Condition: ambient temperature, protect from light and moisture
Shipping Condition: ambient temperature
Product DescriptionQuin 2 forms a stable fluorescent complex with calcium (logKCaY=7.1), but not with magnesium (logMgY=2.7). The complex has a high quantum yield (0.14) at emission wavelength 525 nm, excitation wavelength 339 nm, and emission wavelength 492 nm. Quin 2-AM is an acetoxymethyl ester derivative of Quin 2 that is readily permeable through cell membranes. Within the cell, the ester is hydrolyzed to Quin 2.
1. R. Y. Tsien, New Calcium Indicators and Buffers with High Selectivity against Magnesium and Protons: Design, Synthesis, and Properties of Prototype Structures. Biochemistry. 1980;19:2396-2404.
2. R. Y. Tsien, et al., Calcium Homeostasis in Intact Lymphocytes: Cytoplasmic Free Calcium Monitored with a New, Intracellularly Trapped Fluorescent Indicator. J Cell Biol. 1982;94:325-334.
3. M. B. Feinstein, et al., The Cytoplasmic Concentration of Free Calcium Inplatelets Is Controlled by Stimulators of Cyclic AMP Production (PGD2, PGE1, FORSKOLIN). Biochem Biophys Res Commun. 1983;113:598-604.
4. N. Miyoshi, et al., A New Method of Determining Intracellular Free Ca2+ Concentration Using Quin2-fluorescence. Photochem Photobiol. 1991;53:415-418.