| n-Decyl-β-D-maltoside | :200 mg x 1 |
| n-Dodecyl-β-D-maltoside | :200 mg x 1 |
| n-Octyl-β-D-glucoside | :200 mg x 1 |
n-Octyl-β-D-maltoside
| :200 mg x 1 |
| MEGA-10 | :200 mg x 1 |
Storage Condition: 0-5ºC
Shipping Condition: ambient temperatureProduct Description
Detergent Screening Sets are used to find the best detergent for an application, such as enzyme stabilization, specific membrane protein isolation, assay development, protein crystallization, protein refolding, and so on. Detergent Screening Set (first choice-II) contains commonly used detergents for membrane protein isolation. Detergent Screening Set (for crystallization) contains detergents commonly used for protein crystallization.
Introduction
Introduction
The
phospholipid bilayer is the basic structure of the cell membrane. The
most important functions of cells include transportation of substances,
energy exchange, and transmission of information. These functions are
conducted at the cell membrane by membrane proteins. In membrane
biochemistry research, membrane proteins are solubilized and purified to
study their structure and function. Proteins bound
to cell membranes have hydrophobic sites buried within the phospholipid
bilayers and hydrophilic sites facing toward the water layer.
Detergents are used to isolate large insoluble molecules such as
proteins. Detergents interact with the hydrophobic sites of proteins,
which are then solubilized in the water layer, thus separating membrane
proteins. It is important to choose a detergent that does not disrupt
the bioactivities of target proteins. A detergent requires the following
characteristics to be suitable for isolation of
membrane proteins:
1. Sufficient protein solubilization capability
2. No denaturing or inactivation of proteins
3. No interference with protein activities
4. No precipitation at 4ºC
5. Appropriate critical micelle concentrations (CMC) and micelle size
6. No absorption in the UV region
7. No toxicity
8. Availability of detergent detection methods
9. Non-ionic detergent if ion exchange chromatography is used
In
the past, polyoxyethylene ether non-ionic detergents were widely used.
These detergents, however, had several problems, such as denaturation of
proteins and low CMC value, which cannot be separated easily by
dialysis. n-Octyl-β-D-glucoside, n-Octyl-β-Dthioglucoside, CHAPS, and
CHAPSO eliminate these problems and are widely used today. Most of the
current detergents are non-ionic and easily applied to ion exchange
chromatography purification. deoxy-BIGCHAP is a non-ionic detergent
possessing deoxycholic acid and a gluconamide polar group. It has a high
CMC value of 1.4 mM and can be easily separated by dialysis. Because
its UV absorbance is low, it can be used for the determination of
proteins. deoxy-BIGCHAP has been used for the extraction of opioid
receptors from neuroblastoma or hybrid cells of glyoma. It has also been
applied to adenylate cyclase or acetyltransferase. These detergents are
also widely used to solubilize chromophores or to stabilize enzymes in
diagnostic analyses and biochemical assays.
Trials
of various kinds of detergents are needed to find the appropriate
detergent for each study. Dojindo’s Detergent Screening Sets, which
contain assorted packages of detergents, are available for use in these
trials.