Chemical Name: 1,2-Diamino-4,5-dimethoxybenzene, dihydrochloride
Appearance: white or slightly pink crystalline powder
Purity: ≥98.0% (Titration)
MW: 241.11, C8H14Cl2N2O2Storage Condition: -20°C, protect from moisture
Shipping Condition: blue ice
DDB reacts with aromatic aldehydes in acidic conditions to give strong fluorescent benzimidazole. This reagent, as well as DTAN, can be used for the detection of aromatic aldehydes. DDB is soluble in aqueous solutions. The excitation and emission wavelength of the labeled materials are 338 nm and 402 nm, respectively.
1. M. Nakamura, et al., Fluorimetric Determination of Aromatic Aldehydes with 4, 5-Dimethoxy-1, 2-Diaminobenzene. Anal Chim Acta. 1982;134:39-45.
2. M. Nakamura, et al., Fluorescent Product in the Determination of Aromatic Aldehydes with 4,5-Dimethoxy-1,2-Diaminobenzene. Chem Pharm Bull. 1983;31:2910-2912.
3. S. Hara, et al., Highly Sensitive Determination of N-Acetyl- and N-Glycolylneuraminic Acids in Human Serum and Urine and Rat Serum by Reversed-Phase Liquid Chromatography with Fluorescence Detection. J Chromatogr. 1986;377:111-119.