Application: Thiol compound derivatization for HPLC analysis

Chemical Name: N-(9-Acridinyl)maleimide
CAS: 49759-20-8

Yellow crystalline powder
Purity: ≥96.0% (HPLC)
274.27, C17H10N2O2

Storage Condition: 0-5ºC
Shipping Condition: ambient temperature

Derivatization Reaction

Product Description
NAM is a labeling reagent for sulfhydryl groups that forms highly fluorescent compounds. It reacts with thiol compounds in aqueous solutions at pH 3-10, and can be used to detect picomolar levels of cysteine per injection by HPLC. The sensitivity of NAM is approximately 100 times that of DTNB. The excitation and emission wavelength of the derivatized compound are 365 nm and 435-440 nm, respectively.

1. Y. Nara, et al., Synthesis of N-(9-Acridinyl)maleimide, a Fluorometrical Reagent for Thiol Compounds. Agric Biol Chem. 1978;42:793-798.
2. T. Yagi, et al., A Sensitive Method for the Detection of Aspartate: 2-Oxoglutarate Aminotransferase Activity on Polyacrylmide Gels. Anal Biochem. 1981;110:146-149.
3. H. Meguro, et al., A Novel Fluorometry of Sulfite with High Specificity. Anal Lett. 1983;16:1625-1632.
4. H. Tanno, et al., Assay of L-Methionineγ-Lyase with N-(9-Acridinyl)Maleimide. Agric Biol Chem. 1985;49:1517-1518.
5. K. Akasaka, et al., A Fluorometric Assay of L-Aspartate:2-Oxo-Glutarate Aminotransferase in Human Serum with N-(9-Acridinyl)Maleimide. Anal Lett. 1985;18:357-368.
6. K. Akasaka, et al., Fluorometric Determination of Sulfite with N-(9-Acridinyl)-Maleimide for High Performance Liquid Chromatography. Agric Biol Chem. 1986;50:1139-1144.
7. K. Akasaka, et al., Fluorometric Determination of Sulfites in Rainwater. Anal Sci. 1986;2:443-446.
8. K. Akasaka, et al., 9-Bromomethylacridine a Novel Fluorescent Labeling Reagent of Carboxylic Group for HPLC. Anal Lett. 1987;20:1581-1594.
9. K. Akasaka, et al., Fluorometric Determination of Sulfite in Wine by N-(9-Acridinyl)Maleimide. Agric Biol Chem. 1990;54:501-504.
10. T. Kamata, et al., A Sensitive Fluorometric Assay of Glutathione Reductase Activity with N-(9-Acridinyl)Maleimide. Anal Sci. 1993;9:867-870.
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