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a)Piranha solution: sulfuric acid and 30% hydrogen peroxide, 3:1. Piranha solution is a strong oxidizing agent. Extreme care is necessary when using it.Do not apply Piranha solution to resin-coated plates; it may erode the resin.b)To prepare a SAM-coated plate with the best performance, aminoalkanethiol concentration and soaking time should be individually determined.
a)Irradiation time: 1-1.5 hoursb)SPDP: N-succinimidyl 3-(2-pyridyldithio)propionate. Dissolve SPDP in DMSO to prepare 50 mM solution. Dilute it 25 times with 100 mM triethanolamine buffer, pH 7.0.c)Dissolve thiol-DNA with 100 mM triethanolamine buffer, pH 8.0.