Chemical Description: 1-Methoxy-5-methylphenazinium methylsulfate
Appearance: dark red or reddish-purple powder
Purity (Absorbance): ≥95.0%
Molar absorptivity: ≥2,700 (around 505 nm)
MW: 336.36, C15H16N2O5S
Storage Condition : ambient temperature, protect from moisture
Shipping Condition: ambient temperature
1-Methoxy PMS is easily dissolved by water and alcohol. Its redox potential is +63 mV. Though phenazinium methylsulfate (PMS) is commonly used as an electron carrier for NADH-tetrazolium, the stability of PMS is very poor. However, 1-Methoxy PMS solution can be stored at room temperature for over 3 months without protection from light. An electron of NAD(P)H is transfered by 1-Methoxy PMS to tetrazolium salt (Reaction Scheme). Therefore, it is a useful reagent for NAD(P)H-tetrazolium-based assay systems.
1. R. Hisada, et al., 1-methoxy-5-methylphenazinium Methyl Sulfate. J Biochem. 1977;82:1469-1473.
2. S. Nakamura, et al., Use of 1-methoxy-5-methylphenazinium Methyl Sulfate(1-methoxyPMS) in the Assay of Some Enzymes of Diagnosticimportance. Clin Chim Acta. 1980;101:321-326.
3. C. J. Van Noorden, et al., The Role of Exogenous Electron Carriers in NAD(P)-dependent Dehydrogenase Cytochemistry Studied in vitro and with a Model System of Polyacrylamide Films. J Histochem Cytochem. 1982;30:12-20.
4. H. Tsuge, et al., Partial Purification and Property of Pyridoxine (Pyridoxamine)-5 E phosphate Oxidase Isozymes from Wheat Seedlings. Arch Biochem Biophys. 1982;217:479-484.
5. M. Rabinovitch, et al., Destruction of Leishmania Mexicana Amazonensis Amastigotes within Macrophages in Culture by Phenazine Methosulfate and Other Electron Carriers. J Exp Med. 1982;155:415-431.