Application: Blocking agent for immunostaining with Anti-Nitroguanosine
                       Antibody, standard of 8-Nitroguanosine EIA


Appearance: Pale yellow powder
Purity: ≥90.0% (HPLC)
MW: 196.12, C5H4N6O3

Storage Condition: 0-5°C
Shipping Condition: ambient temperature

Product Description
8-Nitroguanine is a nitrated base of DNA and RNA. It is formed by peroxynitrite, which is generated from nitric oxide and superoxide anion radical. It is known that a large amount of nitric oxide molecules and superoxide anion, generated by inflammation, causes nitration of guanosine. Since chemically modified nucleotides cause mutation during DNA replication, 8-Nitroguanine is thought to be a marker of DNA damage related to mutation and cancer. 8-Nitroguanine (lyophilized) is made by the lyophilization of its phosphate-buffered saline solution and is used in immunohistochemistry for absorption testing. Adding 0.4 ml of distilled water to the 8-Nitroguanine powder produces a 1.2 mmol / l of 8-Nitroguanine solution, which is stable for one month at 4°C. If an antibody pre-treated with excessive 8-Nitroguanine shows negative staining, then the subsequent positive staining with this antibody will be specific for 8-nitroguanine or 8-nitroguanosine formed in DNA or RNA.

Chemical Structure

4-PDS: 1. R. E. Humphrey, et al., Anal. Chem., 42, 698 (1970);
J. Haladjian, et al., J. Electroanal. Chem., 352, 329 (1993);
A. Andersson, et al., Clin. Chem., 39, 1590 (1993);
V. P. Benov, et al., Anal. Lett., 26, 2061 (1993);
T. Kohzuma, et al., Chem Lett., 12, 2029 (1993);
A. Mosca, et al., J. Vac. Sci. Technol., B12, 1486 (1994);
K. Hasinaka, et al., J. Immunol. Methods, 172, 179 (1994);
A. Cricenti, et al., J. Vac. Sci. Technol., B12, 1494 (1994);
G. Pitari, et al., Eur. J. Biochem., 226, 81 (1994);
D. Hobara, et al., Colloids Surf., A93, 241 (1994);
M. Le, G. E. Means, Anal. Biochem., 229, 264 (1995);
S. Cai, et al., J. Membr. Biol., 158, 147 (1997);
K. Cai, et al., Biochemistry, 38, 7925 (1999);
J. Klein-Seetharaman, et al., Biochemistry, 38, 7938 (1999).
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