Chemical Name: 3,3'-{[3,3'-Dimethyl-(1,1'-biphenyl)-4,4'-diyl]bis(azo)}-bis(5-amino-4-hydroxy-2,7-naphthalenedisulfonic acid), tetrasodium salt
CAS: 72-57-1

Blackish brown crystalline powder
MW: 960.81, C34H24N6Na4O14S4

Storage Condition : ambient temperature
Shipping Condition : ambient temperature

Product Description
Trypan Blue is commonly utilized for dead cell staining, in what is called the dye exclusion test. Viable cells are not stained by Trypan Blue. Therefore, dead Trypan Blue-stained cells are easily recognized by microscopy and can be counted using a hematocytometer.Erythrosin B, negrosine, eosin Y, AO and EB are also utilized for this purpose. Though it is hard to detect cells in early to middle stages of apoptosis, Trypan Blue staining is a very simple and widely used method to visualize dead cells.

Chemical Structure

1. K. H. Jones, et al., An Improved Method to Determine Cell Viability by Simultaneous Staining with Fluorescein Diacetate-Propidium Iodide. J Histochem Cytochem. 1985;33:77-79.
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