Chemical Name: N-Succinimidyl 3-(2-pyridyldithio)propionate
Appearance: Amine and sulfhydryl group cross-linking
Purity: ≥98.0% (HPLC)
MW: 312.37, C12H12N2O4S2
Storage Condition: 0-5ºC,
Shipping Condition: ambient temperature
SPDP, a hetero-bifunctional reagent, is useful for bridging two different proteins, such as enzymes and antibodies. SPDP first reacts with a protein molecule through its amino groups. SPDP introduces a pyridyl disulfide moiety to the protein, which is then reduced by DTT to form thiol groups. These thiol groups then form a disulfide bond with another protein molecule thus yielding a heterodimer protein.
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